[2018-10-12 22:29:48] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-12 22:29:48] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-12 22:29:48] Checking for Bowtie index files (genome).. [2018-10-12 22:29:48] Checking for reference FASTA file [2018-10-12 22:29:48] Generating SAM header for Bowtie2Index/genome [2018-10-12 22:29:50] Reading known junctions from GTF file [2018-10-12 22:29:52] Preparing reads left reads: min. length=100, max. length=100, 221728 kept reads (141 discarded) right reads: min. length=100, max. length=100, 221544 kept reads (325 discarded) [2018-10-12 22:29:59] Building transcriptome data files /scratch/8792796.1.p16/tophat2/tmp/RefSeq_GeneBody [2018-10-12 22:30:08] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-12 22:34:50] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 22:35:03] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 22:35:18] Resuming TopHat pipeline with unmapped reads [2018-10-12 22:35:18] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 22:35:33] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 22:35:36] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 22:35:41] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 22:35:45] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 22:35:49] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 22:36:04] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 22:36:08] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 22:36:13] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 22:36:17] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 22:36:21] Searching for junctions via segment mapping [2018-10-12 22:37:35] Retrieving sequences for splices [2018-10-12 22:38:42] Indexing splices [2018-10-12 22:38:53] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 22:38:55] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 22:38:57] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 22:38:59] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 22:39:02] Joining segment hits [2018-10-12 22:40:10] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 22:40:12] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 22:40:15] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 22:40:17] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 22:40:19] Joining segment hits [2018-10-12 22:41:29] Reporting output tracks ----------------------------------------------- [2018-10-12 22:42:58] A summary of the alignment counts can be found in /scratch/8792796.1.p16/tophat2/align_summary.txt [2018-10-12 22:42:58] Run complete: 00:13:09 elapsed