[2018-10-13 07:06:15] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 07:06:15] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 07:06:15] Checking for Bowtie index files (genome).. [2018-10-13 07:06:15] Checking for reference FASTA file [2018-10-13 07:06:15] Generating SAM header for Bowtie2Index/genome [2018-10-13 07:06:17] Reading known junctions from GTF file [2018-10-13 07:06:19] Preparing reads left reads: min. length=100, max. length=100, 1041270 kept reads (78 discarded) right reads: min. length=100, max. length=100, 1040869 kept reads (479 discarded) [2018-10-13 07:06:49] Building transcriptome data files /scratch/8793078.1.p16/tophat2/tmp/RefSeq_GeneBody [2018-10-13 07:07:00] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 07:11:44] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 07:12:12] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 07:12:42] Resuming TopHat pipeline with unmapped reads [2018-10-13 07:12:42] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 07:12:58] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 07:13:02] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 07:13:07] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 07:13:12] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 07:13:17] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 07:13:36] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 07:13:41] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 07:13:47] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 07:13:53] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 07:13:58] Searching for junctions via segment mapping [2018-10-13 07:15:53] Retrieving sequences for splices [2018-10-13 07:16:59] Indexing splices [2018-10-13 07:17:11] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 07:17:13] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 07:17:16] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 07:17:19] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 07:17:21] Joining segment hits [2018-10-13 07:18:37] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 07:18:40] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 07:18:43] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 07:18:46] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 07:18:48] Joining segment hits [2018-10-13 07:20:04] Reporting output tracks ----------------------------------------------- [2018-10-13 07:24:41] A summary of the alignment counts can be found in /scratch/8793078.1.p16/tophat2/align_summary.txt [2018-10-13 07:24:41] Run complete: 00:18:26 elapsed