[2018-10-12 22:29:53] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-12 22:29:53] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-12 22:29:53] Checking for Bowtie index files (genome).. [2018-10-12 22:29:53] Checking for reference FASTA file [2018-10-12 22:29:53] Generating SAM header for Bowtie2Index/genome [2018-10-12 22:29:57] Reading known junctions from GTF file [2018-10-12 22:30:01] Preparing reads left reads: min. length=100, max. length=100, 340458 kept reads (225 discarded) right reads: min. length=100, max. length=100, 340186 kept reads (497 discarded) [2018-10-12 22:30:16] Building transcriptome data files /scratch/8792794.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-12 22:30:33] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-12 22:38:21] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 22:38:52] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 22:39:24] Resuming TopHat pipeline with unmapped reads [2018-10-12 22:39:24] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 22:39:55] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 22:40:04] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 22:40:15] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 22:40:25] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 22:40:34] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 22:41:06] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 22:41:16] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 22:41:29] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 22:41:38] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 22:41:48] Searching for junctions via segment mapping [2018-10-12 22:44:24] Retrieving sequences for splices [2018-10-12 22:46:42] Indexing splices [2018-10-12 22:47:01] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 22:47:05] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 22:47:10] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 22:47:14] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 22:47:19] Joining segment hits [2018-10-12 22:49:35] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 22:49:40] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 22:49:44] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 22:49:48] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 22:49:52] Joining segment hits [2018-10-12 22:52:10] Reporting output tracks ----------------------------------------------- [2018-10-12 22:55:28] A summary of the alignment counts can be found in /scratch/8792794.1.linga/tophat2/align_summary.txt [2018-10-12 22:55:28] Run complete: 00:25:34 elapsed