[2018-10-12 22:27:05] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-12 22:27:05] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-12 22:27:05] Checking for Bowtie index files (genome).. [2018-10-12 22:27:05] Checking for reference FASTA file [2018-10-12 22:27:05] Generating SAM header for Bowtie2Index/genome [2018-10-12 22:27:07] Reading known junctions from GTF file [2018-10-12 22:27:10] Preparing reads left reads: min. length=100, max. length=100, 493311 kept reads (217 discarded) right reads: min. length=100, max. length=100, 492954 kept reads (574 discarded) [2018-10-12 22:27:22] Building transcriptome data files /scratch/8792792.1.p16/tophat2/tmp/RefSeq_GeneBody [2018-10-12 22:27:31] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-12 22:32:15] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 22:32:39] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 22:33:05] Resuming TopHat pipeline with unmapped reads [2018-10-12 22:33:05] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 22:33:30] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 22:33:35] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 22:33:41] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 22:33:46] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 22:33:51] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 22:34:18] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 22:34:23] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 22:34:29] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 22:34:35] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 22:34:40] Searching for junctions via segment mapping [2018-10-12 22:36:08] Retrieving sequences for splices [2018-10-12 22:37:14] Indexing splices [2018-10-12 22:37:25] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 22:37:27] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 22:37:30] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 22:37:32] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 22:37:34] Joining segment hits [2018-10-12 22:38:51] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 22:38:54] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 22:38:56] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 22:38:59] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 22:39:01] Joining segment hits [2018-10-12 22:40:18] Reporting output tracks ----------------------------------------------- [2018-10-12 22:42:26] A summary of the alignment counts can be found in /scratch/8792792.1.p16/tophat2/align_summary.txt [2018-10-12 22:42:26] Run complete: 00:15:21 elapsed