[2018-10-12 22:21:28] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-12 22:21:28] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-12 22:21:28] Checking for Bowtie index files (genome).. [2018-10-12 22:21:28] Checking for reference FASTA file [2018-10-12 22:21:28] Generating SAM header for Bowtie2Index/genome [2018-10-12 22:21:33] Reading known junctions from GTF file [2018-10-12 22:21:38] Preparing reads left reads: min. length=100, max. length=100, 267520 kept reads (260 discarded) right reads: min. length=100, max. length=100, 267266 kept reads (514 discarded) [2018-10-12 22:21:50] Building transcriptome data files /scratch/8792791.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-12 22:22:12] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-12 22:30:36] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 22:31:13] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 22:31:51] Resuming TopHat pipeline with unmapped reads [2018-10-12 22:31:51] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 22:32:34] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 22:32:44] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 22:32:57] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 22:33:08] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 22:33:18] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 22:34:00] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 22:34:09] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 22:34:23] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 22:34:33] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 22:34:45] Searching for junctions via segment mapping [2018-10-12 22:37:20] Retrieving sequences for splices [2018-10-12 22:39:36] Indexing splices [2018-10-12 22:40:00] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 22:40:04] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 22:40:08] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 22:40:12] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 22:40:16] Joining segment hits [2018-10-12 22:42:40] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 22:42:44] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 22:42:48] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 22:42:53] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 22:42:57] Joining segment hits [2018-10-12 22:45:19] Reporting output tracks ----------------------------------------------- [2018-10-12 22:48:32] A summary of the alignment counts can be found in /scratch/8792791.1.linga/tophat2/align_summary.txt [2018-10-12 22:48:32] Run complete: 00:27:04 elapsed