[2018-10-12 22:18:58] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-12 22:18:58] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-12 22:18:58] Checking for Bowtie index files (genome).. [2018-10-12 22:18:58] Checking for reference FASTA file [2018-10-12 22:18:58] Generating SAM header for Bowtie2Index/genome [2018-10-12 22:19:02] Reading known junctions from GTF file [2018-10-12 22:19:06] Preparing reads left reads: min. length=100, max. length=100, 298153 kept reads (201 discarded) right reads: min. length=100, max. length=100, 297938 kept reads (416 discarded) [2018-10-12 22:19:20] Building transcriptome data files /scratch/8792790.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-12 22:19:40] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-12 22:27:55] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 22:28:28] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 22:29:00] Resuming TopHat pipeline with unmapped reads [2018-10-12 22:29:00] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 22:29:35] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 22:29:43] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 22:29:55] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 22:30:04] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 22:30:13] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 22:30:49] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 22:30:59] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 22:31:11] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 22:31:21] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 22:31:30] Searching for junctions via segment mapping [2018-10-12 22:34:02] Retrieving sequences for splices [2018-10-12 22:36:13] Indexing splices [2018-10-12 22:36:33] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 22:36:37] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 22:36:42] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 22:36:46] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 22:36:50] Joining segment hits [2018-10-12 22:39:05] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 22:39:09] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 22:39:14] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 22:39:18] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 22:39:22] Joining segment hits [2018-10-12 22:41:39] Reporting output tracks ----------------------------------------------- [2018-10-12 22:44:46] A summary of the alignment counts can be found in /scratch/8792790.1.linga/tophat2/align_summary.txt [2018-10-12 22:44:46] Run complete: 00:25:48 elapsed