[2018-10-13 16:56:32] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 16:56:32] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 16:56:32] Checking for Bowtie index files (genome).. [2018-10-13 16:56:32] Checking for reference FASTA file [2018-10-13 16:56:32] Generating SAM header for Bowtie2Index/genome [2018-10-13 16:56:37] Reading known junctions from GTF file [2018-10-13 16:56:43] Preparing reads left reads: min. length=100, max. length=100, 229375 kept reads (209 discarded) right reads: min. length=100, max. length=100, 229188 kept reads (396 discarded) [2018-10-13 16:56:53] Building transcriptome data files /scratch/8793376.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 16:57:14] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 17:05:43] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 17:06:06] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 17:06:29] Resuming TopHat pipeline with unmapped reads [2018-10-13 17:06:29] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 17:06:49] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 17:06:56] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 17:07:06] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 17:07:16] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 17:07:25] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 17:07:45] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 17:07:53] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 17:08:03] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 17:08:13] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 17:08:21] Searching for junctions via segment mapping [2018-10-13 17:11:00] Retrieving sequences for splices [2018-10-13 17:13:11] Indexing splices [2018-10-13 17:13:31] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 17:13:35] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 17:13:40] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 17:13:44] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 17:13:48] Joining segment hits [2018-10-13 17:16:57] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 17:17:01] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 17:17:06] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 17:17:10] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 17:17:15] Joining segment hits [2018-10-13 17:19:27] Reporting output tracks ----------------------------------------------- [2018-10-13 17:22:27] A summary of the alignment counts can be found in /scratch/8793376.1.linga/tophat2/align_summary.txt [2018-10-13 17:22:27] Run complete: 00:25:54 elapsed