[2018-10-12 22:13:53] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-12 22:13:53] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-12 22:13:53] Checking for Bowtie index files (genome).. [2018-10-12 22:13:53] Checking for reference FASTA file [2018-10-12 22:13:53] Generating SAM header for Bowtie2Index/genome [2018-10-12 22:13:55] Reading known junctions from GTF file [2018-10-12 22:13:57] Preparing reads left reads: min. length=100, max. length=100, 200528 kept reads (239 discarded) right reads: min. length=100, max. length=100, 200410 kept reads (357 discarded) [2018-10-12 22:14:02] Building transcriptome data files /scratch/8792787.1.p8/tophat2/tmp/RefSeq_GeneBody [2018-10-12 22:14:13] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-12 22:18:55] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 22:19:09] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 22:19:23] Resuming TopHat pipeline with unmapped reads [2018-10-12 22:19:23] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 22:19:41] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 22:19:45] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 22:19:50] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 22:19:54] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 22:19:58] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 22:20:17] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 22:20:21] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 22:20:26] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 22:20:30] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 22:20:34] Searching for junctions via segment mapping [2018-10-12 22:21:47] Retrieving sequences for splices [2018-10-12 22:22:54] Indexing splices [2018-10-12 22:23:05] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 22:23:07] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 22:23:09] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 22:23:11] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 22:23:13] Joining segment hits [2018-10-12 22:24:22] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 22:24:25] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 22:24:27] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 22:24:29] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 22:24:31] Joining segment hits [2018-10-12 22:25:40] Reporting output tracks ----------------------------------------------- [2018-10-12 22:27:14] A summary of the alignment counts can be found in /scratch/8792787.1.p8/tophat2/align_summary.txt [2018-10-12 22:27:14] Run complete: 00:13:21 elapsed