[2018-10-13 06:49:10] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 06:49:10] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 06:49:10] Checking for Bowtie index files (genome).. [2018-10-13 06:49:10] Checking for reference FASTA file [2018-10-13 06:49:10] Generating SAM header for Bowtie2Index/genome [2018-10-13 06:49:14] Reading known junctions from GTF file [2018-10-13 06:49:18] Preparing reads left reads: min. length=100, max. length=100, 948783 kept reads (89 discarded) right reads: min. length=100, max. length=100, 948501 kept reads (371 discarded) [2018-10-13 06:49:57] Building transcriptome data files /scratch/8793066.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 06:50:16] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 06:58:47] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 06:59:34] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 07:00:21] Resuming TopHat pipeline with unmapped reads [2018-10-13 07:00:21] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 07:00:54] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 07:01:03] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 07:01:18] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 07:01:28] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 07:01:38] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 07:02:13] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 07:02:22] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 07:02:37] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 07:02:48] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 07:02:59] Searching for junctions via segment mapping [2018-10-13 07:06:14] Retrieving sequences for splices [2018-10-13 07:08:13] Indexing splices [2018-10-13 07:08:32] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 07:08:37] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 07:08:42] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 07:08:47] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 07:08:51] Joining segment hits [2018-10-13 07:11:07] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 07:11:12] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 07:11:17] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 07:11:22] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 07:11:27] Joining segment hits [2018-10-13 07:13:43] Reporting output tracks ----------------------------------------------- [2018-10-13 07:18:41] A summary of the alignment counts can be found in /scratch/8793066.1.linga/tophat2/align_summary.txt [2018-10-13 07:18:41] Run complete: 00:29:31 elapsed