[2018-10-13 06:37:41] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 06:37:41] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 06:37:41] Checking for Bowtie index files (genome).. [2018-10-13 06:37:41] Checking for reference FASTA file [2018-10-13 06:37:41] Generating SAM header for Bowtie2Index/genome [2018-10-13 06:37:45] Reading known junctions from GTF file [2018-10-13 06:37:49] Preparing reads left reads: min. length=100, max. length=100, 1315788 kept reads (318 discarded) right reads: min. length=100, max. length=100, 1315360 kept reads (746 discarded) [2018-10-13 06:38:43] Building transcriptome data files /scratch/8793060.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 06:39:03] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 06:47:07] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 06:50:38] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 06:54:02] Resuming TopHat pipeline with unmapped reads [2018-10-13 06:54:02] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 06:54:51] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 06:55:01] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 06:55:25] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 06:55:42] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 06:55:59] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 06:56:47] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 06:56:58] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 06:57:23] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 06:57:40] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 06:57:59] Searching for junctions via segment mapping [2018-10-13 07:09:26] Retrieving sequences for splices [2018-10-13 07:11:37] Indexing splices [2018-10-13 07:12:03] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 07:12:12] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 07:12:27] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 07:12:40] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 07:12:56] Joining segment hits [2018-10-13 07:15:31] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 07:15:41] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 07:15:57] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 07:16:11] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 07:16:29] Joining segment hits [2018-10-13 07:19:02] Reporting output tracks ----------------------------------------------- [2018-10-13 08:00:43] A summary of the alignment counts can be found in /scratch/8793060.1.linga/tophat2/align_summary.txt [2018-10-13 08:00:43] Run complete: 01:23:02 elapsed