[2018-10-13 06:37:41] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 06:37:41] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 06:37:41] Checking for Bowtie index files (genome).. [2018-10-13 06:37:41] Checking for reference FASTA file [2018-10-13 06:37:41] Generating SAM header for Bowtie2Index/genome [2018-10-13 06:37:45] Reading known junctions from GTF file [2018-10-13 06:37:49] Preparing reads left reads: min. length=100, max. length=100, 1336351 kept reads (137 discarded) right reads: min. length=100, max. length=100, 1336062 kept reads (426 discarded) [2018-10-13 06:38:44] Building transcriptome data files /scratch/8793059.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 06:39:05] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 06:47:36] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 06:49:45] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 06:51:55] Resuming TopHat pipeline with unmapped reads [2018-10-13 06:51:55] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 06:52:58] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 06:53:11] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 06:53:37] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 06:53:52] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 06:54:06] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 06:55:06] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 06:55:20] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 06:55:45] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 06:56:00] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 06:56:16] Searching for junctions via segment mapping [2018-10-13 07:05:43] Retrieving sequences for splices [2018-10-13 07:08:00] Indexing splices [2018-10-13 07:08:26] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 07:08:37] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 07:08:54] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 07:09:06] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 07:09:16] Joining segment hits [2018-10-13 07:11:51] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 07:12:02] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 07:12:17] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 07:12:30] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 07:12:41] Joining segment hits [2018-10-13 07:15:17] Reporting output tracks ----------------------------------------------- [2018-10-13 07:40:35] A summary of the alignment counts can be found in /scratch/8793059.1.linga/tophat2/align_summary.txt [2018-10-13 07:40:35] Run complete: 01:02:54 elapsed