[2018-10-13 06:15:17] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 06:15:17] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 06:15:17] Checking for Bowtie index files (genome).. [2018-10-13 06:15:17] Checking for reference FASTA file [2018-10-13 06:15:17] Generating SAM header for Bowtie2Index/genome [2018-10-13 06:15:19] Reading known junctions from GTF file [2018-10-13 06:15:21] Preparing reads left reads: min. length=100, max. length=100, 550551 kept reads (156 discarded) right reads: min. length=100, max. length=100, 550391 kept reads (316 discarded) [2018-10-13 06:15:34] Building transcriptome data files /scratch/8793051.1.p8/tophat2/tmp/RefSeq_GeneBody [2018-10-13 06:15:44] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 06:20:26] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 06:20:58] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 06:21:29] Resuming TopHat pipeline with unmapped reads [2018-10-13 06:21:29] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 06:21:44] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 06:21:48] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 06:21:54] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 06:21:59] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 06:22:04] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 06:22:18] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 06:22:23] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 06:22:29] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 06:22:34] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 06:22:40] Searching for junctions via segment mapping [2018-10-13 06:25:12] Retrieving sequences for splices [2018-10-13 06:26:22] Indexing splices [2018-10-13 06:26:33] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 06:26:36] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 06:26:39] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 06:26:43] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 06:26:46] Joining segment hits [2018-10-13 06:28:00] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 06:28:03] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 06:28:06] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 06:28:09] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 06:28:13] Joining segment hits [2018-10-13 06:29:27] Reporting output tracks ----------------------------------------------- [2018-10-13 06:36:16] A summary of the alignment counts can be found in /scratch/8793051.1.p8/tophat2/align_summary.txt [2018-10-13 06:36:16] Run complete: 00:20:58 elapsed