[2018-10-12 22:06:44] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-12 22:06:44] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-12 22:06:44] Checking for Bowtie index files (genome).. [2018-10-12 22:06:44] Checking for reference FASTA file [2018-10-12 22:06:44] Generating SAM header for Bowtie2Index/genome [2018-10-12 22:06:46] Reading known junctions from GTF file [2018-10-12 22:06:49] Preparing reads left reads: min. length=100, max. length=100, 620584 kept reads (432 discarded) right reads: min. length=100, max. length=100, 620087 kept reads (929 discarded) [2018-10-12 22:07:06] Building transcriptome data files /scratch/8792783.1.p16/tophat2/tmp/RefSeq_GeneBody [2018-10-12 22:07:16] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-12 22:11:59] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 22:12:33] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 22:13:09] Resuming TopHat pipeline with unmapped reads [2018-10-12 22:13:09] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 22:13:44] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 22:13:49] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 22:13:57] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 22:14:03] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 22:14:09] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 22:14:45] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 22:14:51] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 22:14:59] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 22:15:06] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 22:15:12] Searching for junctions via segment mapping [2018-10-12 22:16:41] Retrieving sequences for splices [2018-10-12 22:17:48] Indexing splices [2018-10-12 22:17:58] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 22:18:00] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 22:18:03] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 22:18:06] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 22:18:08] Joining segment hits [2018-10-12 22:19:25] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 22:19:28] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 22:19:30] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 22:19:33] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 22:19:35] Joining segment hits [2018-10-12 22:20:53] Reporting output tracks ----------------------------------------------- [2018-10-12 22:23:12] A summary of the alignment counts can be found in /scratch/8792783.1.p16/tophat2/align_summary.txt [2018-10-12 22:23:12] Run complete: 00:16:27 elapsed