[2018-10-13 06:06:46] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 06:06:46] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 06:06:46] Checking for Bowtie index files (genome).. [2018-10-13 06:06:46] Checking for reference FASTA file [2018-10-13 06:06:46] Generating SAM header for Bowtie2Index/genome [2018-10-13 06:06:50] Reading known junctions from GTF file [2018-10-13 06:06:55] Preparing reads left reads: min. length=100, max. length=100, 306833 kept reads (227 discarded) right reads: min. length=100, max. length=100, 306441 kept reads (619 discarded) [2018-10-13 06:07:09] Building transcriptome data files /scratch/8793045.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 06:07:28] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 06:16:09] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 06:16:35] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 06:17:02] Resuming TopHat pipeline with unmapped reads [2018-10-13 06:17:02] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 06:17:22] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 06:17:30] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 06:17:40] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 06:17:49] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 06:17:58] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 06:18:20] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 06:18:28] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 06:18:39] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 06:18:48] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 06:18:58] Searching for junctions via segment mapping [2018-10-13 06:21:49] Retrieving sequences for splices [2018-10-13 06:23:59] Indexing splices [2018-10-13 06:24:18] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 06:24:22] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 06:24:27] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 06:24:31] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 06:24:35] Joining segment hits [2018-10-13 06:27:05] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 06:27:09] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 06:27:14] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 06:27:18] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 06:27:22] Joining segment hits [2018-10-13 06:29:45] Reporting output tracks ----------------------------------------------- [2018-10-13 06:33:46] A summary of the alignment counts can be found in /scratch/8793045.1.linga/tophat2/align_summary.txt [2018-10-13 06:33:46] Run complete: 00:26:59 elapsed