[2018-10-13 05:55:20] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 05:55:20] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 05:55:20] Checking for Bowtie index files (genome).. [2018-10-13 05:55:20] Checking for reference FASTA file [2018-10-13 05:55:20] Generating SAM header for Bowtie2Index/genome [2018-10-13 05:55:25] Reading known junctions from GTF file [2018-10-13 05:55:30] Preparing reads left reads: min. length=100, max. length=100, 1135899 kept reads (285 discarded) right reads: min. length=100, max. length=100, 1135581 kept reads (603 discarded) [2018-10-13 05:56:16] Building transcriptome data files /scratch/8793037.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 05:56:36] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 06:05:33] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 06:07:31] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 06:09:27] Resuming TopHat pipeline with unmapped reads [2018-10-13 06:09:27] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 06:10:20] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 06:10:32] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 06:10:58] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 06:11:14] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 06:11:29] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 06:12:24] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 06:12:36] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 06:13:03] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 06:13:20] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 06:13:37] Searching for junctions via segment mapping [2018-10-13 06:24:23] Retrieving sequences for splices [2018-10-13 06:26:36] Indexing splices [2018-10-13 06:27:02] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 06:27:13] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 06:27:29] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 06:27:42] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 06:27:54] Joining segment hits [2018-10-13 06:30:41] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 06:30:54] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 06:31:12] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 06:31:28] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 06:31:42] Joining segment hits [2018-10-13 06:34:41] Reporting output tracks ----------------------------------------------- [2018-10-13 07:01:45] A summary of the alignment counts can be found in /scratch/8793037.1.linga/tophat2/align_summary.txt [2018-10-13 07:01:45] Run complete: 01:06:24 elapsed