[2018-10-13 05:53:20] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 05:53:20] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 05:53:20] Checking for Bowtie index files (genome).. [2018-10-13 05:53:20] Checking for reference FASTA file [2018-10-13 05:53:20] Generating SAM header for Bowtie2Index/genome [2018-10-13 05:53:24] Reading known junctions from GTF file [2018-10-13 05:53:28] Preparing reads left reads: min. length=100, max. length=100, 1189074 kept reads (86 discarded) right reads: min. length=100, max. length=100, 1188699 kept reads (461 discarded) [2018-10-13 05:54:16] Building transcriptome data files /scratch/8793035.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 05:54:34] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 06:02:53] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 06:03:47] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 06:04:44] Resuming TopHat pipeline with unmapped reads [2018-10-13 06:04:45] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 06:05:12] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 06:05:21] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 06:05:32] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 06:05:42] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 06:05:52] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 06:06:25] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 06:06:34] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 06:06:47] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 06:06:58] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 06:07:09] Searching for junctions via segment mapping [2018-10-13 06:11:06] Retrieving sequences for splices [2018-10-13 06:13:06] Indexing splices [2018-10-13 06:13:30] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 06:13:35] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 06:13:42] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 06:13:48] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 06:13:53] Joining segment hits [2018-10-13 06:16:12] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 06:16:18] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 06:16:24] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 06:16:30] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 06:16:37] Joining segment hits [2018-10-13 06:19:00] Reporting output tracks ----------------------------------------------- [2018-10-13 06:28:39] A summary of the alignment counts can be found in /scratch/8793035.1.linga/tophat2/align_summary.txt [2018-10-13 06:28:39] Run complete: 00:35:19 elapsed