[2018-10-13 05:43:31] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 05:43:31] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 05:43:31] Checking for Bowtie index files (genome).. [2018-10-13 05:43:31] Checking for reference FASTA file [2018-10-13 05:43:31] Generating SAM header for Bowtie2Index/genome [2018-10-13 05:43:35] Reading known junctions from GTF file [2018-10-13 05:43:40] Preparing reads left reads: min. length=100, max. length=100, 1380635 kept reads (196 discarded) right reads: min. length=100, max. length=100, 1380184 kept reads (647 discarded) [2018-10-13 05:44:46] Building transcriptome data files /scratch/8793031.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 05:45:06] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 05:53:47] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 05:55:50] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 05:57:59] Resuming TopHat pipeline with unmapped reads [2018-10-13 05:57:59] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 05:58:53] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 05:59:05] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 05:59:31] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 05:59:46] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 06:00:00] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 06:00:54] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 06:01:08] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 06:01:34] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 06:01:50] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 06:02:05] Searching for junctions via segment mapping [2018-10-13 06:11:12] Retrieving sequences for splices [2018-10-13 06:13:21] Indexing splices [2018-10-13 06:13:45] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 06:13:56] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 06:14:09] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 06:14:19] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 06:14:29] Joining segment hits [2018-10-13 06:17:06] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 06:17:18] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 06:17:32] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 06:17:43] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 06:17:53] Joining segment hits [2018-10-13 06:20:30] Reporting output tracks ----------------------------------------------- [2018-10-13 06:44:50] A summary of the alignment counts can be found in /scratch/8793031.1.linga/tophat2/align_summary.txt [2018-10-13 06:44:50] Run complete: 01:01:19 elapsed