[2018-10-12 22:01:58] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-12 22:01:58] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-12 22:01:58] Checking for Bowtie index files (genome).. [2018-10-12 22:01:58] Checking for reference FASTA file [2018-10-12 22:01:58] Generating SAM header for Bowtie2Index/genome [2018-10-12 22:02:02] Reading known junctions from GTF file [2018-10-12 22:02:07] Preparing reads left reads: min. length=100, max. length=100, 378084 kept reads (260 discarded) right reads: min. length=100, max. length=100, 377767 kept reads (577 discarded) [2018-10-12 22:02:25] Building transcriptome data files /scratch/8792780.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-12 22:02:44] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-12 22:10:55] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 22:11:37] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 22:12:22] Resuming TopHat pipeline with unmapped reads [2018-10-12 22:12:22] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 22:13:08] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 22:13:18] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 22:13:31] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 22:13:42] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 22:13:52] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 22:14:38] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 22:14:48] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 22:15:02] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 22:15:13] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 22:15:24] Searching for junctions via segment mapping [2018-10-12 22:18:17] Retrieving sequences for splices [2018-10-12 22:20:30] Indexing splices [2018-10-12 22:20:52] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 22:20:56] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 22:21:01] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 22:21:05] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 22:21:09] Joining segment hits [2018-10-12 22:23:32] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 22:23:36] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 22:23:41] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 22:23:46] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 22:23:50] Joining segment hits [2018-10-12 22:26:13] Reporting output tracks ----------------------------------------------- [2018-10-12 22:29:58] A summary of the alignment counts can be found in /scratch/8792780.1.linga/tophat2/align_summary.txt [2018-10-12 22:29:58] Run complete: 00:28:00 elapsed