[2018-10-12 22:01:58] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-12 22:01:58] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-12 22:01:58] Checking for Bowtie index files (genome).. [2018-10-12 22:01:58] Checking for reference FASTA file [2018-10-12 22:01:58] Generating SAM header for Bowtie2Index/genome [2018-10-12 22:02:02] Reading known junctions from GTF file [2018-10-12 22:02:07] Preparing reads left reads: min. length=100, max. length=100, 415488 kept reads (306 discarded) right reads: min. length=100, max. length=100, 415111 kept reads (683 discarded) [2018-10-12 22:02:26] Building transcriptome data files /scratch/8792778.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-12 22:02:47] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-12 22:11:56] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 22:12:44] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 22:13:34] Resuming TopHat pipeline with unmapped reads [2018-10-12 22:13:34] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 22:14:27] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 22:14:37] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 22:14:52] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 22:15:02] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 22:15:13] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 22:16:08] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 22:16:18] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 22:16:33] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 22:16:45] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 22:16:57] Searching for junctions via segment mapping [2018-10-12 22:19:44] Retrieving sequences for splices [2018-10-12 22:21:58] Indexing splices [2018-10-12 22:22:21] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 22:22:26] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 22:22:31] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 22:22:36] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 22:22:40] Joining segment hits [2018-10-12 22:25:03] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 22:25:07] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 22:25:12] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 22:25:17] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 22:25:21] Joining segment hits [2018-10-12 22:27:54] Reporting output tracks ----------------------------------------------- [2018-10-12 22:31:35] A summary of the alignment counts can be found in /scratch/8792778.1.linga/tophat2/align_summary.txt [2018-10-12 22:31:35] Run complete: 00:29:36 elapsed