[2018-10-13 05:22:09] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 05:22:09] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 05:22:09] Checking for Bowtie index files (genome).. [2018-10-13 05:22:09] Checking for reference FASTA file [2018-10-13 05:22:09] Generating SAM header for Bowtie2Index/genome [2018-10-13 05:22:11] Reading known junctions from GTF file [2018-10-13 05:22:13] Preparing reads left reads: min. length=100, max. length=100, 924062 kept reads (115 discarded) right reads: min. length=100, max. length=100, 923873 kept reads (304 discarded) [2018-10-13 05:22:39] Building transcriptome data files /scratch/8793023.1.p16/tophat2/tmp/RefSeq_GeneBody [2018-10-13 05:22:51] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 05:27:32] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 05:28:01] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 05:28:30] Resuming TopHat pipeline with unmapped reads [2018-10-13 05:28:30] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 05:28:46] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 05:28:50] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 05:28:56] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 05:29:01] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 05:29:06] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 05:29:24] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 05:29:28] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 05:29:34] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 05:29:40] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 05:29:45] Searching for junctions via segment mapping [2018-10-13 05:31:57] Retrieving sequences for splices [2018-10-13 05:33:04] Indexing splices [2018-10-13 05:33:15] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 05:33:18] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 05:33:21] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 05:33:24] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 05:33:26] Joining segment hits [2018-10-13 05:34:41] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 05:34:44] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 05:34:47] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 05:34:50] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 05:34:52] Joining segment hits [2018-10-13 05:36:08] Reporting output tracks ----------------------------------------------- [2018-10-13 05:41:09] A summary of the alignment counts can be found in /scratch/8793023.1.p16/tophat2/align_summary.txt [2018-10-13 05:41:09] Run complete: 00:19:00 elapsed