[2018-10-13 05:05:15] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 05:05:15] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 05:05:15] Checking for Bowtie index files (genome).. [2018-10-13 05:05:15] Checking for reference FASTA file [2018-10-13 05:05:15] Generating SAM header for Bowtie2Index/genome [2018-10-13 05:05:17] Reading known junctions from GTF file [2018-10-13 05:05:20] Preparing reads left reads: min. length=100, max. length=100, 612586 kept reads (148 discarded) right reads: min. length=100, max. length=100, 612423 kept reads (311 discarded) [2018-10-13 05:05:35] Building transcriptome data files /scratch/8793013.1.p8/tophat2/tmp/RefSeq_GeneBody [2018-10-13 05:05:45] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 05:10:27] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 05:11:10] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 05:11:53] Resuming TopHat pipeline with unmapped reads [2018-10-13 05:11:53] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 05:12:08] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 05:12:13] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 05:12:19] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 05:12:25] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 05:12:31] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 05:12:47] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 05:12:52] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 05:12:58] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 05:13:04] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 05:13:10] Searching for junctions via segment mapping [2018-10-13 05:16:20] Retrieving sequences for splices [2018-10-13 05:17:27] Indexing splices [2018-10-13 05:17:40] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 05:17:43] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 05:17:47] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 05:17:52] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 05:17:56] Joining segment hits [2018-10-13 05:19:12] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 05:19:15] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 05:19:19] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 05:19:24] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 05:19:29] Joining segment hits [2018-10-13 05:20:44] Reporting output tracks ----------------------------------------------- [2018-10-13 05:31:30] A summary of the alignment counts can be found in /scratch/8793013.1.p8/tophat2/align_summary.txt [2018-10-13 05:31:30] Run complete: 00:26:14 elapsed