[2018-10-13 04:49:03] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 04:49:03] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 04:49:03] Checking for Bowtie index files (genome).. [2018-10-13 04:49:03] Checking for reference FASTA file [2018-10-13 04:49:03] Generating SAM header for Bowtie2Index/genome [2018-10-13 04:49:08] Reading known junctions from GTF file [2018-10-13 04:49:12] Preparing reads left reads: min. length=100, max. length=100, 1207431 kept reads (300 discarded) right reads: min. length=100, max. length=100, 1207097 kept reads (634 discarded) [2018-10-13 04:50:00] Building transcriptome data files /scratch/8793009.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 04:50:18] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 04:58:25] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 05:01:08] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 05:03:40] Resuming TopHat pipeline with unmapped reads [2018-10-13 05:03:41] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 05:04:31] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 05:04:45] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 05:05:15] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 05:05:33] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 05:05:50] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 05:06:44] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 05:06:59] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 05:07:29] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 05:07:51] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 05:08:11] Searching for junctions via segment mapping [2018-10-13 05:21:00] Retrieving sequences for splices [2018-10-13 05:23:04] Indexing splices [2018-10-13 05:23:30] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 05:23:44] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 05:24:10] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 05:24:29] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 05:24:43] Joining segment hits [2018-10-13 05:27:13] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 05:27:28] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 05:27:54] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 05:28:14] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 05:28:30] Joining segment hits [2018-10-13 05:31:06] Reporting output tracks ----------------------------------------------- [2018-10-13 06:08:08] A summary of the alignment counts can be found in /scratch/8793009.1.linga/tophat2/align_summary.txt [2018-10-13 06:08:08] Run complete: 01:19:04 elapsed