[2018-10-13 04:49:03] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 04:49:03] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 04:49:04] Checking for Bowtie index files (genome).. [2018-10-13 04:49:04] Checking for reference FASTA file [2018-10-13 04:49:04] Generating SAM header for Bowtie2Index/genome [2018-10-13 04:49:08] Reading known junctions from GTF file [2018-10-13 04:49:13] Preparing reads left reads: min. length=100, max. length=100, 532458 kept reads (178 discarded) right reads: min. length=100, max. length=100, 532165 kept reads (471 discarded) [2018-10-13 04:49:42] Building transcriptome data files /scratch/8793007.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 04:50:02] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 04:58:54] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 04:59:35] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 05:00:16] Resuming TopHat pipeline with unmapped reads [2018-10-13 05:00:16] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 05:00:46] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 05:00:55] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 05:01:07] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 05:01:18] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 05:01:28] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 05:01:59] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 05:02:08] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 05:02:20] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 05:02:31] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 05:02:42] Searching for junctions via segment mapping [2018-10-13 05:06:52] Retrieving sequences for splices [2018-10-13 05:09:03] Indexing splices [2018-10-13 05:09:23] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 05:09:28] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 05:09:34] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 05:09:39] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 05:09:45] Joining segment hits [2018-10-13 05:12:14] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 05:12:19] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 05:12:24] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 05:12:30] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 05:12:35] Joining segment hits [2018-10-13 05:15:03] Reporting output tracks ----------------------------------------------- [2018-10-13 05:21:45] A summary of the alignment counts can be found in /scratch/8793007.1.linga/tophat2/align_summary.txt [2018-10-13 05:21:45] Run complete: 00:32:41 elapsed