[2018-10-13 04:49:04] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 04:49:04] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 04:49:04] Checking for Bowtie index files (genome).. [2018-10-13 04:49:04] Checking for reference FASTA file [2018-10-13 04:49:04] Generating SAM header for Bowtie2Index/genome [2018-10-13 04:49:08] Reading known junctions from GTF file [2018-10-13 04:49:13] Preparing reads left reads: min. length=100, max. length=100, 551527 kept reads (365 discarded) right reads: min. length=100, max. length=100, 551105 kept reads (787 discarded) [2018-10-13 04:49:35] Building transcriptome data files /scratch/8793005.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 04:49:56] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 04:58:51] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 04:59:58] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 05:01:13] Resuming TopHat pipeline with unmapped reads [2018-10-13 05:01:13] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 05:01:52] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 05:02:02] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 05:02:18] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 05:02:31] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 05:02:44] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 05:03:22] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 05:03:32] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 05:03:49] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 05:04:01] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 05:04:14] Searching for junctions via segment mapping [2018-10-13 05:10:22] Retrieving sequences for splices [2018-10-13 05:12:28] Indexing splices [2018-10-13 05:12:52] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 05:12:57] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 05:13:06] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 05:13:14] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 05:13:22] Joining segment hits [2018-10-13 05:15:44] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 05:15:50] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 05:15:59] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 05:16:07] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 05:16:16] Joining segment hits [2018-10-13 05:18:37] Reporting output tracks ----------------------------------------------- [2018-10-13 05:34:34] A summary of the alignment counts can be found in /scratch/8793005.1.linga/tophat2/align_summary.txt [2018-10-13 05:34:34] Run complete: 00:45:29 elapsed