[2018-10-13 05:15:31] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 05:15:31] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 05:15:31] Checking for Bowtie index files (genome).. [2018-10-13 05:15:31] Checking for reference FASTA file [2018-10-13 05:15:31] Generating SAM header for Bowtie2Index/genome [2018-10-13 05:15:35] Reading known junctions from GTF file [2018-10-13 05:15:39] Preparing reads left reads: min. length=100, max. length=100, 953816 kept reads (130 discarded) right reads: min. length=100, max. length=100, 953538 kept reads (408 discarded) [2018-10-13 05:16:19] Building transcriptome data files /scratch/8793018.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 05:16:38] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 05:24:37] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 05:26:05] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 05:27:37] Resuming TopHat pipeline with unmapped reads [2018-10-13 05:27:37] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 05:28:20] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 05:28:30] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 05:28:49] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 05:29:02] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 05:29:15] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 05:30:02] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 05:30:13] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 05:30:33] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 05:30:46] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 05:31:00] Searching for junctions via segment mapping [2018-10-13 05:38:51] Retrieving sequences for splices [2018-10-13 05:41:03] Indexing splices [2018-10-13 05:41:32] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 05:41:40] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 05:41:52] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 05:42:03] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 05:42:15] Joining segment hits [2018-10-13 05:44:49] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 05:44:57] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 05:45:08] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 05:45:19] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 05:45:29] Joining segment hits [2018-10-13 05:48:03] Reporting output tracks ----------------------------------------------- [2018-10-13 06:08:57] A summary of the alignment counts can be found in /scratch/8793018.1.linga/tophat2/align_summary.txt [2018-10-13 06:08:57] Run complete: 00:53:26 elapsed