[2018-10-12 22:01:57] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-12 22:01:57] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-12 22:01:57] Checking for Bowtie index files (genome).. [2018-10-12 22:01:57] Checking for reference FASTA file [2018-10-12 22:01:57] Generating SAM header for Bowtie2Index/genome [2018-10-12 22:02:01] Reading known junctions from GTF file [2018-10-12 22:02:06] Preparing reads left reads: min. length=100, max. length=100, 320716 kept reads (227 discarded) right reads: min. length=100, max. length=100, 320459 kept reads (484 discarded) [2018-10-12 22:02:20] Building transcriptome data files /scratch/8792777.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-12 22:02:40] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-12 22:10:58] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 22:11:31] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 22:12:05] Resuming TopHat pipeline with unmapped reads [2018-10-12 22:12:05] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 22:12:45] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 22:12:55] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 22:13:06] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 22:13:17] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 22:13:26] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 22:14:06] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 22:14:16] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 22:14:30] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 22:14:40] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 22:14:50] Searching for junctions via segment mapping [2018-10-12 22:17:23] Retrieving sequences for splices [2018-10-12 22:19:29] Indexing splices [2018-10-12 22:19:47] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 22:19:51] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 22:19:56] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 22:20:01] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 22:20:05] Joining segment hits [2018-10-12 22:22:22] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 22:22:26] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 22:22:31] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 22:22:35] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 22:22:39] Joining segment hits [2018-10-12 22:24:52] Reporting output tracks ----------------------------------------------- [2018-10-12 22:28:12] A summary of the alignment counts can be found in /scratch/8792777.1.linga/tophat2/align_summary.txt [2018-10-12 22:28:12] Run complete: 00:26:15 elapsed