[2018-10-13 04:45:31] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 04:45:31] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 04:45:31] Checking for Bowtie index files (genome).. [2018-10-13 04:45:31] Checking for reference FASTA file [2018-10-13 04:45:31] Generating SAM header for Bowtie2Index/genome [2018-10-13 04:45:35] Reading known junctions from GTF file [2018-10-13 04:45:39] Preparing reads left reads: min. length=100, max. length=100, 614762 kept reads (83 discarded) right reads: min. length=100, max. length=100, 614569 kept reads (276 discarded) [2018-10-13 04:46:06] Building transcriptome data files /scratch/8793001.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 04:46:26] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 04:54:36] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 04:55:15] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 04:55:54] Resuming TopHat pipeline with unmapped reads [2018-10-13 04:55:54] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 04:56:16] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 04:56:24] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 04:56:35] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 04:56:44] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 04:56:53] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 04:57:15] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 04:57:23] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 04:57:34] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 04:57:43] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 04:57:52] Searching for junctions via segment mapping [2018-10-13 05:01:36] Retrieving sequences for splices [2018-10-13 05:03:45] Indexing splices [2018-10-13 05:04:06] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 05:04:10] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 05:04:16] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 05:04:21] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 05:04:26] Joining segment hits [2018-10-13 05:06:52] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 05:06:57] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 05:07:03] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 05:07:08] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 05:07:13] Joining segment hits [2018-10-13 05:09:41] Reporting output tracks ----------------------------------------------- [2018-10-13 05:17:30] A summary of the alignment counts can be found in /scratch/8793001.1.linga/tophat2/align_summary.txt [2018-10-13 05:17:30] Run complete: 00:31:59 elapsed