[2018-10-12 21:44:56] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-12 21:44:56] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-12 21:44:56] Checking for Bowtie index files (genome).. [2018-10-12 21:44:56] Checking for reference FASTA file [2018-10-12 21:44:56] Generating SAM header for Bowtie2Index/genome [2018-10-12 21:45:00] Reading known junctions from GTF file [2018-10-12 21:45:05] Preparing reads left reads: min. length=100, max. length=100, 159510 kept reads (97 discarded) right reads: min. length=100, max. length=100, 159367 kept reads (240 discarded) [2018-10-12 21:45:12] Building transcriptome data files /scratch/8792769.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-12 21:45:31] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-12 21:53:50] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 21:54:12] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 21:54:34] Resuming TopHat pipeline with unmapped reads [2018-10-12 21:54:34] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 21:55:03] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 21:55:11] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 21:55:21] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 21:55:30] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 21:55:38] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 21:56:07] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 21:56:16] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 21:56:26] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 21:56:35] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 21:56:44] Searching for junctions via segment mapping [2018-10-12 21:59:07] Retrieving sequences for splices [2018-10-12 22:01:16] Indexing splices [2018-10-12 22:01:35] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 22:01:38] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 22:01:43] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 22:01:47] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 22:01:51] Joining segment hits [2018-10-12 22:04:04] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 22:04:08] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 22:04:12] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 22:04:16] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 22:04:20] Joining segment hits [2018-10-12 22:06:35] Reporting output tracks ----------------------------------------------- [2018-10-12 22:09:18] A summary of the alignment counts can be found in /scratch/8792769.1.linga/tophat2/align_summary.txt [2018-10-12 22:09:18] Run complete: 00:24:21 elapsed