[2018-10-13 04:17:32] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 04:17:32] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 04:17:33] Checking for Bowtie index files (genome).. [2018-10-13 04:17:33] Checking for reference FASTA file [2018-10-13 04:17:33] Generating SAM header for Bowtie2Index/genome [2018-10-13 04:17:38] Reading known junctions from GTF file [2018-10-13 04:17:42] Preparing reads left reads: min. length=100, max. length=100, 705403 kept reads (63 discarded) right reads: min. length=100, max. length=100, 705030 kept reads (436 discarded) [2018-10-13 04:18:10] Building transcriptome data files /scratch/8792988.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 04:18:29] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 04:26:22] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 04:26:57] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 04:27:32] Resuming TopHat pipeline with unmapped reads [2018-10-13 04:27:32] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 04:28:45] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 04:29:02] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 04:29:26] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 04:29:44] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 04:30:04] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 04:31:16] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 04:31:32] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 04:31:52] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 04:32:09] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 04:32:27] Searching for junctions via segment mapping [2018-10-13 04:35:36] Retrieving sequences for splices [2018-10-13 04:37:40] Indexing splices [2018-10-13 04:38:00] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 04:38:06] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 04:38:12] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 04:38:19] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 04:38:25] Joining segment hits [2018-10-13 04:40:44] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 04:40:50] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 04:40:56] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 04:41:02] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 04:41:08] Joining segment hits [2018-10-13 04:44:04] Reporting output tracks ----------------------------------------------- [2018-10-13 04:47:28] A summary of the alignment counts can be found in /scratch/8792988.1.linga/tophat2/align_summary.txt [2018-10-13 04:47:28] Run complete: 00:29:56 elapsed