[2018-10-13 04:14:33] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 04:14:33] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 04:14:33] Checking for Bowtie index files (genome).. [2018-10-13 04:14:33] Checking for reference FASTA file [2018-10-13 04:14:33] Generating SAM header for Bowtie2Index/genome [2018-10-13 04:14:37] Reading known junctions from GTF file [2018-10-13 04:14:41] Preparing reads left reads: min. length=100, max. length=100, 1125575 kept reads (100 discarded) right reads: min. length=100, max. length=100, 1125093 kept reads (582 discarded) [2018-10-13 04:15:28] Building transcriptome data files /scratch/8792987.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 04:15:47] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 04:23:56] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 04:24:53] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 04:25:48] Resuming TopHat pipeline with unmapped reads [2018-10-13 04:25:48] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 04:26:27] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 04:26:35] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 04:26:48] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 04:26:58] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 04:27:08] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 04:27:49] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 04:27:59] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 04:28:13] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 04:28:25] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 04:28:38] Searching for junctions via segment mapping [2018-10-13 04:32:17] Retrieving sequences for splices [2018-10-13 04:34:28] Indexing splices [2018-10-13 04:34:49] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 04:34:54] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 04:35:01] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 04:35:06] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 04:35:12] Joining segment hits [2018-10-13 04:37:42] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 04:37:48] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 04:37:54] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 04:38:00] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 04:38:06] Joining segment hits [2018-10-13 04:40:36] Reporting output tracks ----------------------------------------------- [2018-10-13 04:50:29] A summary of the alignment counts can be found in /scratch/8792987.1.linga/tophat2/align_summary.txt [2018-10-13 04:50:29] Run complete: 00:35:56 elapsed