[2018-10-13 04:12:12] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 04:12:12] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 04:12:12] Checking for Bowtie index files (genome).. [2018-10-13 04:12:12] Checking for reference FASTA file [2018-10-13 04:12:12] Generating SAM header for Bowtie2Index/genome [2018-10-13 04:12:16] Reading known junctions from GTF file [2018-10-13 04:12:20] Preparing reads left reads: min. length=100, max. length=100, 467458 kept reads (267 discarded) right reads: min. length=100, max. length=100, 467365 kept reads (360 discarded) [2018-10-13 04:12:38] Building transcriptome data files /scratch/8792985.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 04:12:58] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 04:21:42] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 04:22:57] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 04:24:12] Resuming TopHat pipeline with unmapped reads [2018-10-13 04:24:12] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 04:24:41] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 04:24:49] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 04:25:04] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 04:25:16] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 04:25:29] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 04:25:58] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 04:26:06] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 04:26:21] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 04:26:33] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 04:26:47] Searching for junctions via segment mapping [2018-10-13 04:32:40] Retrieving sequences for splices [2018-10-13 04:34:51] Indexing splices [2018-10-13 04:35:15] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 04:35:21] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 04:35:30] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 04:35:38] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 04:35:46] Joining segment hits [2018-10-13 04:38:13] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 04:38:19] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 04:38:28] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 04:38:37] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 04:38:46] Joining segment hits [2018-10-13 04:41:21] Reporting output tracks ----------------------------------------------- [2018-10-13 05:01:07] A summary of the alignment counts can be found in /scratch/8792985.1.linga/tophat2/align_summary.txt [2018-10-13 05:01:07] Run complete: 00:48:55 elapsed