-----------------------
Need shift 1 command (have more than 9 arguments):
-----------------------
-----------------------
Start of variable list:
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Sample_ID:
LZ_M34
Dataset_DIR:
/restricted/projectnb/waxmanlab/kkarri/liver_zonation/infusion
Sample_Labels_DIR:
/restricted/projectnb/waxmanlab/kkarri/liver_zonation/infusion/Scripts/00_Setup_Pipeline
GTF_Files_DIR:
/unprotected/projects/waxmanlab/routines/GTF_Files
MODE:
intersection-nonempty
STRANDEDNESS_HTSeq:
reverse
FEATURE_ID:
gene_id
SCRIPT_DIR:
/restricted/projectnb/waxmanlab/kkarri/liver_zonation/infusion_rerun/Scripts/07a_Extract_Counts_HTSeq
ANNOTATION_FILE:
genes.gtf
FEATURE_TYPE:
exon
-----------------------
End of variable list
-----------------------
==========================================================
Starting on : Sun May 13 14:11:22 EDT 2018
Running on node : scc-kb2
Current directory : /restricted/projectnb/waxmanlab/kkarri/liver_zonation/infusion_rerun/Scripts/07a_Extract_Counts_HTSeq
Current job ID : 6184521
Current job name : Step_07a_LZ_M34
Task index number : undefined
Parameter for multiple cores : 1
==========================================================

Change dir to scratch directory


Print scratch directory location:

/scratch/6184521.1.linga

Loading required modules...


List files in scratch directory:

total 527M
drwx------   2 kkarri waxmanlab 4.0K May 13 14:11 .
drwxrwxrwt. 58 root   root       92K May 13 14:11 ..
-rw-r--r--   1 kkarri waxmanlab    0 May 13 14:11 LZ_M34_HTSeq.out
-rw-r--r--   1 kkarri waxmanlab 447M May 13 14:11 LZ_M34_primary_unique.bam
-rwxr-xr-x   1 kkarri waxmanlab  81M May 13 14:11 genes.gtf

Starting to run my commands


Starting samtools and htseq-count

samtools view LZ_M34'_sorted'.bam | htseq-count --stranded=reverse --type=exon --idattr=gene_id --order=name --mode=intersection-nonempty - genes.gtf  > LZ_M34_HTSeq.out

Ending samtools and htseq-count


List files in scratch

total 1.3G
drwx------   2 kkarri waxmanlab 4.0K May 13 14:17 .
drwxrwxrwt. 49 root   root       92K May 13 14:41 ..
-rw-r--r--   1 kkarri waxmanlab 237K May 13 14:45 LZ_M34_HTSeq.out
-rw-r--r--   1 kkarri waxmanlab 447M May 13 14:11 LZ_M34_primary_unique.bam
-rw-r--r--   1 kkarri waxmanlab 728M May 13 14:17 LZ_M34_sorted.bam
-rwxr-xr-x   1 kkarri waxmanlab  81M May 13 14:11 genes.gtf
==========================================================
Finished on : Sun May 13 14:45:35 EDT 2018
0 hours, 34 minutes and 13 seconds elapsed.
==========================================================