----------------------- Need shift 1 command (have more than 9 arguments): ----------------------- ----------------------- Start of variable list: ----------------------- Sample_ID: LZ_M31 Dataset_DIR: /restricted/projectnb/waxmanlab/kkarri/liver_zonation/infusion Sample_Labels_DIR: /restricted/projectnb/waxmanlab/kkarri/liver_zonation/infusion/Scripts/00_Setup_Pipeline GTF_Files_DIR: /unprotected/projects/waxmanlab/routines/GTF_Files MODE: intersection-nonempty STRANDEDNESS_HTSeq: reverse FEATURE_ID: gene_id SCRIPT_DIR: /restricted/projectnb/waxmanlab/kkarri/liver_zonation/infusion/Scripts/07a_Extract_Counts_HTSeq ANNOTATION_FILE: RefSeq_GeneBody.gtf FEATURE_TYPE: exon ----------------------- End of variable list ----------------------- ========================================================== Starting on : Fri May 11 15:13:38 EDT 2018 Running on node : scc-ka1 Current directory : /restricted/projectnb/waxmanlab/kkarri/liver_zonation/infusion/Scripts/07a_Extract_Counts_HTSeq Current job ID : 6178528 Current job name : Step_07a_LZ_M31 Task index number : undefined Parameter for multiple cores : 1 ========================================================== Change dir to scratch directory Print scratch directory location: /scratch/6178528.1.linga Loading required modules... List files in scratch directory: total 406M drwx------ 2 kkarri waxmanlab 4.0K May 11 15:13 . drwxrwxrwt. 55 root root 124K May 11 15:13 .. -rw-r--r-- 1 kkarri waxmanlab 0 May 11 15:13 LZ_M31_HTSeq.out -rw-r--r-- 1 kkarri waxmanlab 335M May 11 15:13 LZ_M31_primary_unique.bam -rw-r--r-- 1 kkarri waxmanlab 72M May 11 15:13 RefSeq_GeneBody.gtf Starting to run my commands Starting samtools and htseq-count samtools view LZ_M31'_sorted'.bam | htseq-count --stranded=reverse --type=exon --idattr=gene_id --order=name --mode=intersection-nonempty - RefSeq_GeneBody.gtf > LZ_M31_HTSeq.out Ending samtools and htseq-count List files in scratch total 943M drwx------ 2 kkarri waxmanlab 4.0K May 11 15:20 . drwxrwxrwt. 58 root root 124K May 11 15:47 .. -rw-r--r-- 1 kkarri waxmanlab 241K May 11 15:47 LZ_M31_HTSeq.out -rw-r--r-- 1 kkarri waxmanlab 335M May 11 15:13 LZ_M31_primary_unique.bam -rw-r--r-- 1 kkarri waxmanlab 537M May 11 15:20 LZ_M31_sorted.bam -rw-r--r-- 1 kkarri waxmanlab 72M May 11 15:13 RefSeq_GeneBody.gtf ========================================================== Finished on : Fri May 11 15:47:58 EDT 2018 0 hours, 34 minutes and 20 seconds elapsed. ==========================================================