[2018-05-11 14:09:44] Beginning TopHat run (v2.1.1)
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[2018-05-11 14:09:44] Checking for Bowtie
		  Bowtie version:	 2.2.2.0
[2018-05-11 14:09:44] Checking for Bowtie index files (genome)..
[2018-05-11 14:09:44] Checking for reference FASTA file
[2018-05-11 14:09:44] Generating SAM header for Bowtie2Index/genome
[2018-05-11 14:09:46] Reading known junctions from GTF file
[2018-05-11 14:09:49] Preparing reads
	 left reads: min. length=33, max. length=33, 23193647 kept reads (11432 discarded)
[2018-05-11 14:13:15] Building transcriptome data files /scratch/6177268.1.p16/tophat2/tmp/RefSeq_GeneBody
[2018-05-11 14:13:24] Building Bowtie index from RefSeq_GeneBody.fa
[2018-05-11 14:18:16] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 
[2018-05-11 14:24:54] Resuming TopHat pipeline with unmapped reads
Warning: you have only one segment per read.
	If the read length is greater than or equal to 45bp,
	we strongly recommend that you decrease --segment-length to about half the read length because TopHat will work better with multiple segments
[2018-05-11 14:24:54] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 
[2018-05-11 14:27:03] Searching for junctions via segment mapping
[2018-05-11 14:28:07] Retrieving sequences for splices
[2018-05-11 14:29:13] Indexing splices
Building a SMALL index
[2018-05-11 14:29:23] Mapping left_kept_reads.m2g_um_unmapped to genome segment_juncs with Bowtie2 (1/1)
[2018-05-11 14:29:25] Joining segment hits
[2018-05-11 14:33:53] Reporting output tracks
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[2018-05-11 14:40:38] A summary of the alignment counts can be found in /scratch/6177268.1.p16/tophat2/align_summary.txt
[2018-05-11 14:40:38] Run complete: 00:30:53 elapsed
[samopen] SAM header is present: 22 sequences.
[bam_sort_core] merging from 5 files...