[2018-05-13 12:57:40] Beginning TopHat run (v2.1.1)
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[2018-05-13 12:57:40] Checking for Bowtie
		  Bowtie version:	 2.2.2.0
[2018-05-13 12:57:41] Checking for Bowtie index files (genome)..
[2018-05-13 12:57:41] Checking for reference FASTA file
[2018-05-13 12:57:41] Generating SAM header for Bowtie2Index/genome
[2018-05-13 12:57:42] Reading known junctions from GTF file
[2018-05-13 12:57:47] Preparing reads
	 left reads: min. length=33, max. length=33, 23193647 kept reads (11432 discarded)
[2018-05-13 13:01:16] Building transcriptome data files /scratch/6184272.1.p8/tophat2/tmp/RefSeq_GeneBody
[2018-05-13 13:01:26] Building Bowtie index from RefSeq_GeneBody.fa
[2018-05-13 13:06:19] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 
[2018-05-13 13:12:55] Resuming TopHat pipeline with unmapped reads
Warning: you have only one segment per read.
	If the read length is greater than or equal to 45bp,
	we strongly recommend that you decrease --segment-length to about half the read length because TopHat will work better with multiple segments
[2018-05-13 13:12:55] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 
[2018-05-13 13:15:05] Searching for junctions via segment mapping
[2018-05-13 13:16:09] Retrieving sequences for splices
[2018-05-13 13:17:15] Indexing splices
[2018-05-13 13:17:25] Mapping left_kept_reads.m2g_um_unmapped to genome segment_juncs with Bowtie2 (1/1)
open: No such file or directory
Error: bam2fastx failed to open BAM file /scratch/6184272.1.p8/tophat2/tmp/left_kept_reads.m2g_um_unmapped.bam
[2018-05-13 13:17:27] Joining segment hits
[2018-05-13 13:21:55] Reporting output tracks
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[2018-05-13 13:28:38] A summary of the alignment counts can be found in /scratch/6184272.1.p8/tophat2/align_summary.txt
[2018-05-13 13:28:38] Run complete: 00:30:58 elapsed