----------------------- Need shift 1 command (have more than 9 arguments): ----------------------- ----------------------- Start of variable list: ----------------------- Sample_ID: LZ_M11 Dataset_DIR: /restricted/projectnb/waxmanlab/kkarri/liver_zonation Sample_Labels_DIR: /restricted/projectnb/waxmanlab/kkarri/liver_zonation/Scripts/00_Setup_Pipeline GTF_Files_DIR: /unprotected/projects/waxmanlab/routines/GTF_Files MODE: intersection-nonempty STRANDEDNESS_HTSeq: reverse FEATURE_ID: gene_id SCRIPT_DIR: /restricted/projectnb/waxmanlab/kkarri/liver_zonation/Scripts/07a_Extract_Counts_HTSeq ANNOTATION_FILE: genes.gtf FEATURE_TYPE: exon ----------------------- End of variable list ----------------------- ========================================================== Starting on : Wed May 9 18:45:35 EDT 2018 Running on node : scc-kb2 Current directory : /restricted/projectnb/waxmanlab/kkarri/liver_zonation/Scripts/07a_Extract_Counts_HTSeq Current job ID : 6138196 Current job name : Step_07a_LZ_M11 Task index number : undefined Parameter for multiple cores : 1 ========================================================== Change dir to scratch directory Print scratch directory location: /scratch/6138196.1.linga Loading required modules... List files in scratch directory: total 348M drwx------ 2 kkarri waxmanlab 4.0K May 9 18:45 . drwxrwxrwt. 69 root root 92K May 9 18:45 .. -rw-r--r-- 1 kkarri waxmanlab 0 May 9 18:45 LZ_M11_HTSeq.out -rw-r--r-- 1 kkarri waxmanlab 268M May 9 18:45 LZ_M11_primary_unique.bam -rwxr-xr-x 1 kkarri waxmanlab 81M May 9 18:45 genes.gtf Starting to run my commands Starting samtools and htseq-count samtools view LZ_M11'_sorted'.bam | htseq-count --stranded=reverse --type=exon --idattr=gene_id --order=name --mode=intersection-nonempty - genes.gtf > LZ_M11_HTSeq.out Ending samtools and htseq-count List files in scratch total 784M drwx------ 2 kkarri waxmanlab 4.0K May 9 18:50 . drwxrwxrwt. 60 root root 92K May 9 19:11 .. -rw-r--r-- 1 kkarri waxmanlab 234K May 9 19:11 LZ_M11_HTSeq.out -rw-r--r-- 1 kkarri waxmanlab 268M May 9 18:45 LZ_M11_primary_unique.bam -rw-r--r-- 1 kkarri waxmanlab 436M May 9 18:50 LZ_M11_sorted.bam -rwxr-xr-x 1 kkarri waxmanlab 81M May 9 18:45 genes.gtf ========================================================== Finished on : Wed May 9 19:11:33 EDT 2018 0 hours, 25 minutes and 58 seconds elapsed. ==========================================================