----------------------- Need shift 1 command (have more than 9 arguments): ----------------------- ----------------------- Start of variable list: ----------------------- Sample_ID: MM_M3 Dataset_DIR: /restricted/projectnb/waxmanlab/kkarri/samples/fastqfiles/Water_Saline Sample_Labels_DIR: /restricted/projectnb/waxmanlab/kkarri/samples/fastqfiles/Water_Saline/Scripts/00_Setup_Pipeline GTF_Files_DIR: /restricted/projectnb/waxmanlab/kkarri/GTF_Files1 MODE: intersection-nonempty STRANDEDNESS_HTSeq: no FEATURE_ID: gene_id SCRIPT_DIR: /restricted/projectnb/waxmanlab/kkarri/samples/fastqfiles/Water_Saline/Scripts/08a_Extract_Counts_HTSeq ANNOTATION_FILE: RefSeq_GeneBody.gtf FEATURE_TYPE: exon ----------------------- End of variable list ----------------------- ========================================================== Starting on : Sat Jun 17 05:06:48 EDT 2017 Running on node : scc-ka1 Current directory : /restricted/projectnb/waxmanlab/kkarri/samples/fastqfiles/Water_Saline/Scripts/08a_Extract_Counts_HTSeq Current job ID : 9394998 Current job name : Step_08a_MM_M3 Task index number : undefined Parameter for multiple cores : 1 ========================================================== Change dir to scratch directory Print scratch directory location: /scratch/9394998.1.linga Loading required modules... List files in scratch directory: total 2.5G drwxr-xr-x 2 kkarri montilab-p 4.0K Jun 17 05:06 . drwxrwxrwt. 202 root root 124K Jun 17 05:06 .. -rw-r--r-- 1 kkarri montilab-p 0 Jun 17 05:06 MM_M3_HTSeq.out -rw-r--r-- 1 kkarri montilab-p 2.5G Jun 17 05:06 MM_M3_primary_unique.bam -rwx------ 1 kkarri montilab-p 41M Jun 17 05:06 RefSeq_GeneBody.gtf Starting to run my commands Starting samtools and htseq-count samtools view MM_M3'_sorted'.bam | htseq-count --stranded=no --type=exon --idattr=gene_id --order=name --mode=intersection-nonempty - RefSeq_GeneBody.gtf > MM_M3_HTSeq.out Ending samtools and htseq-count List files in scratch total 6.3G drwxr-xr-x 2 kkarri montilab-p 4.0K Jun 17 05:35 . drwxrwxrwt. 199 root root 124K Jun 17 06:31 .. -rw-r--r-- 1 kkarri montilab-p 166K Jun 17 06:42 MM_M3_HTSeq.out -rw-r--r-- 1 kkarri montilab-p 2.5G Jun 17 05:06 MM_M3_primary_unique.bam -rw-r--r-- 1 kkarri montilab-p 3.8G Jun 17 05:34 MM_M3_sorted.bam -rwx------ 1 kkarri montilab-p 41M Jun 17 05:06 RefSeq_GeneBody.gtf ========================================================== Finished on : Sat Jun 17 06:42:18 EDT 2017 1 hours, 35 minutes and 30 seconds elapsed. ==========================================================