[2017-06-16 10:20:08] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2017-06-16 10:20:08] Checking for Bowtie Bowtie version: 2.2.2.0 [2017-06-16 10:20:08] Checking for Bowtie index files (genome).. [2017-06-16 10:20:08] Checking for reference FASTA file [2017-06-16 10:20:08] Generating SAM header for Bowtie2Index/genome [2017-06-16 10:20:14] Reading known junctions from GTF file [2017-06-16 10:20:16] Preparing reads left reads: min. length=101, max. length=101, 17627982 kept reads (43 discarded) right reads: min. length=101, max. length=101, 17583101 kept reads (44924 discarded) [2017-06-16 10:36:48] Building transcriptome data files /scratch/9351459.1.linga/tophat2/tmp/RefSeq_GeneBody [2017-06-16 10:37:03] Building Bowtie index from RefSeq_GeneBody.fa [2017-06-16 10:38:54] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2017-06-16 10:50:08] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2017-06-16 11:01:33] Resuming TopHat pipeline with unmapped reads [2017-06-16 11:01:33] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2017-06-16 11:11:52] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2017-06-16 11:13:46] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2017-06-16 11:15:49] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2017-06-16 11:17:52] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2017-06-16 11:20:15] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2017-06-16 11:33:35] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2017-06-16 11:36:11] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2017-06-16 11:39:12] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2017-06-16 11:42:40] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2017-06-16 11:46:43] Searching for junctions via segment mapping [2017-06-16 12:16:59] Retrieving sequences for splices [2017-06-16 12:19:19] Indexing splices [2017-06-16 12:20:06] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2017-06-16 12:21:33] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2017-06-16 12:23:08] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2017-06-16 12:24:38] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2017-06-16 12:26:17] Joining segment hits [2017-06-16 12:30:32] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2017-06-16 12:32:19] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2017-06-16 12:34:10] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2017-06-16 12:35:58] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2017-06-16 12:37:54] Joining segment hits [2017-06-16 12:42:16] Reporting output tracks ----------------------------------------------- [2017-06-16 13:06:17] A summary of the alignment counts can be found in /scratch/9351459.1.linga/tophat2/align_summary.txt [2017-06-16 13:06:17] Run complete: 02:46:08 elapsed