[2017-06-16 10:19:51] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2017-06-16 10:19:51] Checking for Bowtie Bowtie version: 2.2.2.0 [2017-06-16 10:19:52] Checking for Bowtie index files (genome).. [2017-06-16 10:19:52] Checking for reference FASTA file [2017-06-16 10:19:52] Generating SAM header for Bowtie2Index/genome [2017-06-16 10:20:00] Reading known junctions from GTF file [2017-06-16 10:20:05] Preparing reads left reads: min. length=101, max. length=101, 14229320 kept reads (31 discarded) right reads: min. length=101, max. length=101, 14190142 kept reads (39209 discarded) [2017-06-16 10:33:44] Building transcriptome data files /scratch/9351452.1.linga/tophat2/tmp/RefSeq_GeneBody [2017-06-16 10:34:00] Building Bowtie index from RefSeq_GeneBody.fa [2017-06-16 10:35:58] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2017-06-16 10:44:37] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2017-06-16 10:52:56] Resuming TopHat pipeline with unmapped reads [2017-06-16 10:52:56] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2017-06-16 11:01:59] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2017-06-16 11:03:41] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2017-06-16 11:05:32] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2017-06-16 11:07:26] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2017-06-16 11:09:32] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2017-06-16 11:21:11] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2017-06-16 11:23:46] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2017-06-16 11:26:22] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2017-06-16 11:33:47] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2017-06-16 11:38:11] Searching for junctions via segment mapping [2017-06-16 12:07:29] Retrieving sequences for splices [2017-06-16 12:10:30] Indexing splices [2017-06-16 12:11:15] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2017-06-16 12:12:37] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2017-06-16 12:14:04] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2017-06-16 12:15:22] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2017-06-16 12:16:42] Joining segment hits [2017-06-16 12:20:48] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2017-06-16 12:22:27] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2017-06-16 12:24:00] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2017-06-16 12:25:34] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2017-06-16 12:27:15] Joining segment hits [2017-06-16 12:31:52] Reporting output tracks ----------------------------------------------- [2017-06-16 12:52:18] A summary of the alignment counts can be found in /scratch/9351452.1.linga/tophat2/align_summary.txt [2017-06-16 12:52:18] Run complete: 02:32:27 elapsed