[2017-06-14 21:34:42] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2017-06-14 21:34:42] Checking for Bowtie Bowtie version: 2.2.2.0 [2017-06-14 21:34:43] Checking for Bowtie index files (genome).. [2017-06-14 21:34:43] Checking for reference FASTA file [2017-06-14 21:34:43] Generating SAM header for Bowtie2Index/genome [2017-06-14 21:34:47] Reading known junctions from GTF file [2017-06-14 21:34:50] Preparing reads left reads: min. length=101, max. length=101, 18974336 kept reads (68 discarded) right reads: min. length=101, max. length=101, 18925366 kept reads (49038 discarded) [2017-06-14 21:54:50] Building transcriptome data files /scratch/9328374.1.linga/tophat2/tmp/RefSeq_GeneBody [2017-06-14 21:55:06] Building Bowtie index from RefSeq_GeneBody.fa [2017-06-14 21:56:51] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2017-06-14 22:10:20] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2017-06-14 22:23:12] Resuming TopHat pipeline with unmapped reads [2017-06-14 22:23:12] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2017-06-14 22:35:02] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2017-06-14 22:37:02] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2017-06-14 22:39:20] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2017-06-14 22:41:41] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2017-06-14 22:44:22] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2017-06-14 22:58:12] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2017-06-14 23:01:13] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2017-06-14 23:04:26] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2017-06-14 23:08:14] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2017-06-14 23:12:23] Searching for junctions via segment mapping [2017-06-14 23:29:25] Retrieving sequences for splices [2017-06-14 23:31:46] Indexing splices [2017-06-14 23:32:32] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2017-06-14 23:33:27] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2017-06-14 23:34:33] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2017-06-14 23:35:38] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2017-06-14 23:36:42] Joining segment hits [2017-06-14 23:40:27] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2017-06-14 23:41:37] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2017-06-14 23:43:01] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2017-06-14 23:44:30] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2017-06-14 23:45:54] Joining segment hits [2017-06-14 23:50:02] Reporting output tracks ----------------------------------------------- [2017-06-15 00:16:12] A summary of the alignment counts can be found in /scratch/9328374.1.linga/tophat2/align_summary.txt [2017-06-15 00:16:12] Run complete: 02:41:29 elapsed