################################################################### List of Pipeline Parameters Please check that the following parameters are correct before running the pipeline. 02_Review_Pipeline_Parameters.sh script run date: Tue Jun 21 15:51:49 EDT 2016 Pipeline version: v2.1.0 Refer to Pipeline_Version_History.txt for details. ################################################################### #-------------------------------------------------------------- "Global Variables" = variables used by multiple steps #-------------------------------------------------------------- Dataset_DIR= /projectnb/wax-es/aramp10/G83_Samples BU_User= aramp10 VM_DIR_FASTQC= /home/waxmanlab/public_html/FASTQC/G83_Samples #-------------------------------------------------------------- Check if VM_DIR_FASTQC exists: VM_DIR_FASTQC exists! #-------------------------------------------------------------- Dataset_Label= G83 GTF_Files_DIR= /unprotected/projects/waxmanlab/routines/GTF_Files #-------------------------------------------------------------- "Step-specific Variables" = variables used by a particular step #-------------------------------------------------------------- #--------------------------- Job: TopHat_Paired_End Bowtie2Index_DIR= /projectnb/wax-es/aramp10/Bowtie2 STRANDEDNESS= fr-unstranded BIOANALYZER_LEN= 277 ADAPTOR_LEN= 60 READ_LEN= 99 #--------------------------- Job: CollectRnaSeqMetrics STRAND_SPECIFICITY= NONE #--------------------------- Job: Extract_Counts MODE= intersection-nonempty STRANDEDNESS_HTSeq= no STRANDEDNESS_featureCount= 0 #--------------------------- Job: bamCorrelate zMin= 0.6 zMax 1.0 #--------------------------- Job: UCSC_BigWig STRAND_RULE none #--------------------------- #-------------------------------------------------------------- "Additional Variables" = variables not user-specified #-------------------------------------------------------------- #--------------------------- Job: Extract_Counts FEATURE_ID gene_id #--------------------------- Sample_Labels_DIR /projectnb/wax-es/aramp10/G83_Samples/Scripts/00_Setup_Pipeline SCRIPT_DIR /projectnb/wax-es/aramp10/G83_Samples/Scripts/00_Setup_Pipeline #--------------------------- Job: TopHat_Paired_End FRAGMENT_LEN 157 DISTANCE_BT_READ_PAIR -41 ANNOTATION_FILE RefSeq_GeneBody.gtf #--------------------------- Job: UCSC_BigWig VM_DIR_public_html /home/aramp10/public_html/G83 #-------------------------------------------------------------- Check if VM_DIR_public_html exists: VM_DIR_public_html_FASTQC exists! #-------------------------------------------------------------- #--------------------------- TIME_LIMIT 96:00:00 #-------------------------------------------------------------- DiffExp-specific variables #-------------------------------------------------------------- #--------------------------- Job: 09a_DiffExp_1_HTSeq #--------------------------- CONDITION_1_NAME Female CONDITION_2_NAME Male COMPAR_NUM 1 COUNT_PROGRAM HTSeq #--------------------------- Job: 09b_DiffExp_1_featureCounts #--------------------------- CONDITION_1_NAME Female CONDITION_2_NAME Male COMPAR_NUM 1 COUNT_PROGRAM featureCounts #--------------------------- Job: 09c_DiffExp_1_lncRNA_featureCounts #--------------------------- CONDITION_1_NAME Female CONDITION_2_NAME Male COMPAR_NUM 1 COUNT_PROGRAM featureCounts #-------------------------------------------------------------- Generate_Tracks: No parameters to print. Check that the Sample_Labels.txt has a color column. #-------------------------------------------------------------- ###################################################################