[2017-06-14 21:34:35] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2017-06-14 21:34:35] Checking for Bowtie Bowtie version: 2.2.2.0 [2017-06-14 21:34:35] Checking for Bowtie index files (genome).. [2017-06-14 21:34:35] Checking for reference FASTA file [2017-06-14 21:34:35] Generating SAM header for Bowtie2Index/genome [2017-06-14 21:34:40] Reading known junctions from GTF file [2017-06-14 21:34:43] Preparing reads left reads: min. length=101, max. length=101, 20646293 kept reads (7511 discarded) right reads: min. length=101, max. length=101, 20635670 kept reads (18134 discarded) [2017-06-14 21:56:19] Building transcriptome data files /scratch/9328369.1.linga/tophat2/tmp/RefSeq_GeneBody [2017-06-14 21:56:39] Building Bowtie index from RefSeq_GeneBody.fa [2017-06-14 21:58:39] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2017-06-14 22:13:01] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2017-06-14 22:27:26] Resuming TopHat pipeline with unmapped reads [2017-06-14 22:27:26] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2017-06-14 22:40:38] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2017-06-14 22:42:54] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2017-06-14 22:45:28] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2017-06-14 22:48:10] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2017-06-14 22:51:06] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2017-06-14 23:04:59] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2017-06-14 23:07:15] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2017-06-14 23:09:47] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2017-06-14 23:14:07] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2017-06-14 23:17:41] Searching for junctions via segment mapping [2017-06-14 23:36:43] Retrieving sequences for splices [2017-06-14 23:39:20] Indexing splices [2017-06-14 23:40:17] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2017-06-14 23:41:41] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2017-06-14 23:43:11] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2017-06-14 23:44:25] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2017-06-14 23:45:36] Joining segment hits [2017-06-14 23:49:56] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2017-06-14 23:51:11] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2017-06-14 23:52:32] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2017-06-14 23:53:55] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2017-06-14 23:55:15] Joining segment hits [2017-06-14 23:59:08] Reporting output tracks ----------------------------------------------- [2017-06-15 00:28:12] A summary of the alignment counts can be found in /scratch/9328369.1.linga/tophat2/align_summary.txt [2017-06-15 00:28:12] Run complete: 02:53:36 elapsed