----------------------- Need shift 1 command (have more than 9 arguments): ----------------------- ----------------------- Start of variable list: ----------------------- Sample_ID: NSM_IP1 Dataset_DIR: /restricted/projectnb/waxmanlab/kkarri/samples/fastqfiles/IP_Saline Sample_Labels_DIR: /restricted/projectnb/waxmanlab/kkarri/samples/fastqfiles/IP_Saline/Scripts/00_Setup_Pipeline GTF_Files_DIR: /restricted/projectnb/waxmanlab/kkarri/GTF_Files1 MODE: intersection-nonempty STRANDEDNESS_HTSeq: no FEATURE_ID: gene_id SCRIPT_DIR: /restricted/projectnb/waxmanlab/kkarri/samples/fastqfiles/IP_Saline/Scripts/08a_Extract_Counts_HTSeq ANNOTATION_FILE: genes.gtf FEATURE_TYPE: exon ----------------------- End of variable list ----------------------- ========================================================== Starting on : Thu Jun 15 06:27:17 EDT 2017 Running on node : scc-kb7 Current directory : /restricted/projectnb/waxmanlab/kkarri/samples/fastqfiles/IP_Saline/Scripts/08a_Extract_Counts_HTSeq Current job ID : 9332966 Current job name : Step_08a_NSM_IP1 Task index number : undefined Parameter for multiple cores : 1 ========================================================== Change dir to scratch directory Print scratch directory location: /scratch/9332966.1.linga Loading required modules... List files in scratch directory: total 1.9G drwxr-xr-x 2 kkarri montilab-p 4.0K Jun 15 06:27 . drwxrwxrwt. 85 root root 168K Jun 15 06:27 .. -rw-r--r-- 1 kkarri montilab-p 0 Jun 15 06:27 NSM_IP1_HTSeq.out -rw-r--r-- 1 kkarri montilab-p 1.9G Jun 15 06:27 NSM_IP1_primary_unique.bam -rwxr-xr-x 1 kkarri montilab-p 52M Jun 15 06:27 genes.gtf Starting to run my commands Starting samtools and htseq-count samtools view NSM_IP1'_sorted'.bam | htseq-count --stranded=no --type=exon --idattr=gene_id --order=name --mode=intersection-nonempty - genes.gtf > NSM_IP1_HTSeq.out Ending samtools and htseq-count List files in scratch total 4.6G drwxr-xr-x 2 kkarri montilab-p 4.0K Jun 15 06:44 . drwxrwxrwt. 84 root root 168K Jun 15 07:26 .. -rw-r--r-- 1 kkarri montilab-p 167K Jun 15 07:29 NSM_IP1_HTSeq.out -rw-r--r-- 1 kkarri montilab-p 1.9G Jun 15 06:27 NSM_IP1_primary_unique.bam -rw-r--r-- 1 kkarri montilab-p 2.7G Jun 15 06:44 NSM_IP1_sorted.bam -rwxr-xr-x 1 kkarri montilab-p 52M Jun 15 06:27 genes.gtf ========================================================== Finished on : Thu Jun 15 07:29:46 EDT 2017 1 hours, 2 minutes and 29 seconds elapsed. ==========================================================