[2017-06-14 21:34:42] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2017-06-14 21:34:42] Checking for Bowtie Bowtie version: 2.2.2.0 [2017-06-14 21:34:43] Checking for Bowtie index files (genome).. [2017-06-14 21:34:43] Checking for reference FASTA file [2017-06-14 21:34:43] Generating SAM header for Bowtie2Index/genome [2017-06-14 21:34:48] Reading known junctions from GTF file [2017-06-14 21:34:51] Preparing reads left reads: min. length=101, max. length=101, 19310225 kept reads (57 discarded) right reads: min. length=101, max. length=101, 19261572 kept reads (48710 discarded) [2017-06-14 21:54:51] Building transcriptome data files /scratch/9328375.1.linga/tophat2/tmp/RefSeq_GeneBody [2017-06-14 21:55:07] Building Bowtie index from RefSeq_GeneBody.fa [2017-06-14 21:56:52] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2017-06-14 22:10:15] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2017-06-14 22:23:08] Resuming TopHat pipeline with unmapped reads [2017-06-14 22:23:08] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2017-06-14 22:34:00] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2017-06-14 22:35:51] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2017-06-14 22:37:58] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2017-06-14 22:40:06] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2017-06-14 22:42:31] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2017-06-14 22:57:13] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2017-06-14 23:00:04] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2017-06-14 23:03:17] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2017-06-14 23:07:11] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2017-06-14 23:11:32] Searching for junctions via segment mapping [2017-06-14 23:22:56] Retrieving sequences for splices [2017-06-14 23:25:29] Indexing splices Building a SMALL index [2017-06-14 23:26:20] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2017-06-14 23:27:11] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2017-06-14 23:28:08] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2017-06-14 23:29:07] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2017-06-14 23:30:01] Joining segment hits [2017-06-14 23:33:54] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2017-06-14 23:35:00] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2017-06-14 23:36:13] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2017-06-14 23:37:22] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2017-06-14 23:38:31] Joining segment hits [2017-06-14 23:42:49] Reporting output tracks ----------------------------------------------- [2017-06-15 00:08:28] A summary of the alignment counts can be found in /scratch/9328375.1.linga/tophat2/align_summary.txt [2017-06-15 00:08:28] Run complete: 02:33:45 elapsed [samopen] SAM header is present: 23 sequences. [bam_sort_core] merging from 13 files...