[2017-06-14 21:34:25] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2017-06-14 21:34:25] Checking for Bowtie Bowtie version: 2.2.2.0 [2017-06-14 21:34:25] Checking for Bowtie index files (genome).. [2017-06-14 21:34:25] Checking for reference FASTA file [2017-06-14 21:34:25] Generating SAM header for Bowtie2Index/genome [2017-06-14 21:34:29] Reading known junctions from GTF file [2017-06-14 21:34:32] Preparing reads left reads: min. length=101, max. length=101, 46023193 kept reads (241294 discarded) right reads: min. length=101, max. length=101, 46237018 kept reads (27469 discarded) [2017-06-14 22:28:21] Building transcriptome data files /scratch/9328372.1.linga/tophat2/tmp/RefSeq_GeneBody [2017-06-14 22:28:42] Building Bowtie index from RefSeq_GeneBody.fa [2017-06-14 22:30:47] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2017-06-14 23:03:33] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2017-06-14 23:36:55] Resuming TopHat pipeline with unmapped reads [2017-06-14 23:36:55] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2017-06-15 00:02:09] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2017-06-15 00:06:22] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2017-06-15 00:11:02] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2017-06-15 00:15:45] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2017-06-15 00:21:32] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2017-06-15 00:46:57] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2017-06-15 00:52:09] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2017-06-15 00:58:04] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2017-06-15 01:04:18] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2017-06-15 01:11:25] Searching for junctions via segment mapping [2017-06-15 02:01:20] Retrieving sequences for splices [2017-06-15 02:03:41] Indexing splices [2017-06-15 02:04:47] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2017-06-15 02:08:18] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2017-06-15 02:12:09] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2017-06-15 02:15:35] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2017-06-15 02:19:25] Joining segment hits [2017-06-15 02:24:27] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2017-06-15 02:27:53] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2017-06-15 02:32:00] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2017-06-15 02:36:02] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2017-06-15 02:40:05] Joining segment hits [2017-06-15 02:45:14] Reporting output tracks ----------------------------------------------- [2017-06-15 03:42:17] A summary of the alignment counts can be found in /scratch/9328372.1.linga/tophat2/align_summary.txt [2017-06-15 03:42:17] Run complete: 06:07:51 elapsed