[2018-07-14 20:29:52] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-07-14 20:29:52] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-07-14 20:29:53] Checking for Bowtie index files (genome).. [2018-07-14 20:29:53] Checking for reference FASTA file [2018-07-14 20:29:53] Generating SAM header for Bowtie2Index/genome [2018-07-14 20:29:57] Reading known junctions from GTF file [2018-07-14 20:30:01] Preparing reads left reads: min. length=151, max. length=151, 18298009 kept reads (0 discarded) right reads: min. length=151, max. length=151, 18297654 kept reads (355 discarded) [2018-07-14 20:49:02] Building transcriptome data files /scratch/6936772.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-07-14 20:49:22] Building Bowtie index from RefSeq_GeneBody.fa [2018-07-14 20:57:56] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-07-14 21:14:35] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-07-14 21:31:56] Resuming TopHat pipeline with unmapped reads [2018-07-14 21:31:56] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-07-14 22:03:30] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/6) [2018-07-14 22:13:32] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/6) [2018-07-14 22:24:21] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/6) [2018-07-14 22:34:48] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/6) [2018-07-14 22:43:02] Mapping left_kept_reads.m2g_um_seg5 to genome genome with Bowtie2 (5/6) [2018-07-14 22:47:30] Mapping left_kept_reads.m2g_um_seg6 to genome genome with Bowtie2 (6/6) [2018-07-14 22:51:20] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-07-14 23:27:24] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/6) [2018-07-14 23:37:04] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/6) [2018-07-14 23:48:38] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/6) [2018-07-14 23:58:55] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/6) [2018-07-15 00:08:58] Mapping right_kept_reads.m2g_um_seg5 to genome genome with Bowtie2 (5/6) [2018-07-15 00:16:36] Mapping right_kept_reads.m2g_um_seg6 to genome genome with Bowtie2 (6/6) [2018-07-15 00:21:01] Searching for junctions via segment mapping [2018-07-15 07:19:42] Retrieving sequences for splices [2018-07-15 07:21:56] Indexing splices [2018-07-15 07:22:38] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/6) [2018-07-15 07:30:16] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/6) [2018-07-15 07:37:46] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/6) [2018-07-15 07:45:17] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/6) [2018-07-15 07:51:47] Mapping left_kept_reads.m2g_um_seg5 to genome segment_juncs with Bowtie2 (5/6) [2018-07-15 07:55:51] Mapping left_kept_reads.m2g_um_seg6 to genome segment_juncs with Bowtie2 (6/6) [2018-07-15 07:56:56] Joining segment hits [2018-07-15 08:04:04] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/6) [2018-07-15 08:11:52] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/6) [2018-07-15 08:19:40] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/6) [2018-07-15 08:27:00] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/6) [2018-07-15 08:32:58] Mapping right_kept_reads.m2g_um_seg5 to genome segment_juncs with Bowtie2 (5/6) [2018-07-15 08:36:21] Mapping right_kept_reads.m2g_um_seg6 to genome segment_juncs with Bowtie2 (6/6) [2018-07-15 08:37:17] Joining segment hits [2018-07-15 08:44:15] Reporting output tracks ----------------------------------------------- [2018-07-15 09:22:07] A summary of the alignment counts can be found in /scratch/6936772.1.linga/tophat2/align_summary.txt [2018-07-15 09:22:07] Run complete: 12:52:14 elapsed