[2018-07-14 20:21:14] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-07-14 20:21:14] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-07-14 20:21:15] Checking for Bowtie index files (genome).. [2018-07-14 20:21:15] Checking for reference FASTA file [2018-07-14 20:21:15] Generating SAM header for Bowtie2Index/genome [2018-07-14 20:21:19] Reading known junctions from GTF file [2018-07-14 20:21:23] Preparing reads left reads: min. length=151, max. length=151, 16268058 kept reads (0 discarded) right reads: min. length=151, max. length=151, 16267725 kept reads (333 discarded) [2018-07-14 20:37:02] Building transcriptome data files /scratch/6936770.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-07-14 20:37:20] Building Bowtie index from RefSeq_GeneBody.fa [2018-07-14 20:46:11] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-07-14 21:07:12] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-07-14 21:27:50] Resuming TopHat pipeline with unmapped reads [2018-07-14 21:27:50] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-07-14 21:55:16] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/6) [2018-07-14 22:07:16] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/6) [2018-07-14 22:19:47] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/6) [2018-07-14 22:32:50] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/6) [2018-07-14 22:43:17] Mapping left_kept_reads.m2g_um_seg5 to genome genome with Bowtie2 (5/6) [2018-07-14 22:49:20] Mapping left_kept_reads.m2g_um_seg6 to genome genome with Bowtie2 (6/6) [2018-07-14 22:53:49] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-07-14 23:23:49] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/6) [2018-07-14 23:36:07] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/6) [2018-07-14 23:47:50] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/6) [2018-07-15 00:00:49] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/6) [2018-07-15 00:12:44] Mapping right_kept_reads.m2g_um_seg5 to genome genome with Bowtie2 (5/6) [2018-07-15 00:21:14] Mapping right_kept_reads.m2g_um_seg6 to genome genome with Bowtie2 (6/6) [2018-07-15 00:25:54] Searching for junctions via segment mapping [2018-07-15 12:32:20] Retrieving sequences for splices [2018-07-15 12:34:15] Indexing splices [2018-07-15 12:34:54] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/6) [2018-07-15 12:48:55] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/6) [2018-07-15 13:02:05] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/6) [2018-07-15 13:15:40] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/6) [2018-07-15 13:26:40] Mapping left_kept_reads.m2g_um_seg5 to genome segment_juncs with Bowtie2 (5/6) [2018-07-15 13:33:16] Mapping left_kept_reads.m2g_um_seg6 to genome segment_juncs with Bowtie2 (6/6) [2018-07-15 13:34:39] Joining segment hits [2018-07-15 13:42:10] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/6) [2018-07-15 13:56:30] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/6) [2018-07-15 14:11:24] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/6) [2018-07-15 14:25:37] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/6) [2018-07-15 14:38:07] Mapping right_kept_reads.m2g_um_seg5 to genome segment_juncs with Bowtie2 (5/6) [2018-07-15 14:45:12] Mapping right_kept_reads.m2g_um_seg6 to genome segment_juncs with Bowtie2 (6/6) [2018-07-15 14:46:42] Joining segment hits [2018-07-15 14:54:40] Reporting output tracks ----------------------------------------------- [2018-07-15 15:29:07] A summary of the alignment counts can be found in /scratch/6936770.1.linga/tophat2/align_summary.txt [2018-07-15 15:29:07] Run complete: 19:07:53 elapsed