[2018-07-14 20:21:14] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-07-14 20:21:14] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-07-14 20:21:15] Checking for Bowtie index files (genome).. [2018-07-14 20:21:15] Checking for reference FASTA file [2018-07-14 20:21:15] Generating SAM header for Bowtie2Index/genome [2018-07-14 20:21:19] Reading known junctions from GTF file [2018-07-14 20:21:23] Preparing reads left reads: min. length=151, max. length=151, 17272729 kept reads (0 discarded) right reads: min. length=151, max. length=151, 17272413 kept reads (316 discarded) [2018-07-14 20:42:39] Building transcriptome data files /scratch/6936767.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-07-14 20:42:56] Building Bowtie index from RefSeq_GeneBody.fa [2018-07-14 20:50:44] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-07-14 21:09:33] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-07-14 21:27:53] Resuming TopHat pipeline with unmapped reads [2018-07-14 21:27:53] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-07-14 21:56:14] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/6) [2018-07-14 22:05:27] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/6) [2018-07-14 22:15:07] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/6) [2018-07-14 22:26:25] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/6) [2018-07-14 22:36:45] Mapping left_kept_reads.m2g_um_seg5 to genome genome with Bowtie2 (5/6) [2018-07-14 22:43:28] Mapping left_kept_reads.m2g_um_seg6 to genome genome with Bowtie2 (6/6) [2018-07-14 22:48:58] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-07-14 23:23:24] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/6) [2018-07-14 23:34:03] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/6) [2018-07-14 23:44:07] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/6) [2018-07-14 23:53:33] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/6) [2018-07-15 00:03:06] Mapping right_kept_reads.m2g_um_seg5 to genome genome with Bowtie2 (5/6) [2018-07-15 00:09:36] Mapping right_kept_reads.m2g_um_seg6 to genome genome with Bowtie2 (6/6) [2018-07-15 00:14:42] Searching for junctions via segment mapping [2018-07-15 10:27:29] Retrieving sequences for splices [2018-07-15 10:29:50] Indexing splices [2018-07-15 10:30:37] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/6) [2018-07-15 10:45:00] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/6) [2018-07-15 11:00:02] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/6) [2018-07-15 11:14:32] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/6) [2018-07-15 11:26:37] Mapping left_kept_reads.m2g_um_seg5 to genome segment_juncs with Bowtie2 (5/6) [2018-07-15 11:33:05] Mapping left_kept_reads.m2g_um_seg6 to genome segment_juncs with Bowtie2 (6/6) [2018-07-15 11:34:30] Joining segment hits [2018-07-15 11:41:53] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/6) [2018-07-15 11:56:07] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/6) [2018-07-15 12:11:43] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/6) [2018-07-15 12:27:07] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/6) [2018-07-15 12:40:45] Mapping right_kept_reads.m2g_um_seg5 to genome segment_juncs with Bowtie2 (5/6) [2018-07-15 12:47:29] Mapping right_kept_reads.m2g_um_seg6 to genome segment_juncs with Bowtie2 (6/6) [2018-07-15 12:48:54] Joining segment hits [2018-07-15 12:57:18] Reporting output tracks ----------------------------------------------- [2018-07-15 13:34:21] A summary of the alignment counts can be found in /scratch/6936767.1.linga/tophat2/align_summary.txt [2018-07-15 13:34:21] Run complete: 17:13:06 elapsed