[2018-07-14 20:21:15] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-07-14 20:21:15] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-07-14 20:21:15] Checking for Bowtie index files (genome).. [2018-07-14 20:21:15] Checking for reference FASTA file [2018-07-14 20:21:15] Generating SAM header for Bowtie2Index/genome [2018-07-14 20:21:19] Reading known junctions from GTF file [2018-07-14 20:21:23] Preparing reads left reads: min. length=151, max. length=151, 20703604 kept reads (0 discarded) right reads: min. length=151, max. length=151, 20703231 kept reads (373 discarded) [2018-07-14 20:44:43] Building transcriptome data files /scratch/6936766.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-07-14 20:45:00] Building Bowtie index from RefSeq_GeneBody.fa [2018-07-14 20:53:15] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-07-14 21:15:03] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-07-14 21:37:58] Resuming TopHat pipeline with unmapped reads [2018-07-14 21:37:59] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-07-14 22:12:20] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/6) [2018-07-14 22:25:33] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/6) [2018-07-14 22:39:52] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/6) [2018-07-14 22:54:20] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/6) [2018-07-14 23:05:40] Mapping left_kept_reads.m2g_um_seg5 to genome genome with Bowtie2 (5/6) [2018-07-14 23:12:00] Mapping left_kept_reads.m2g_um_seg6 to genome genome with Bowtie2 (6/6) [2018-07-14 23:16:42] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-07-14 23:56:20] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/6) [2018-07-15 00:10:54] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/6) [2018-07-15 00:24:48] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/6) [2018-07-15 00:40:56] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/6) [2018-07-15 00:56:08] Mapping right_kept_reads.m2g_um_seg5 to genome genome with Bowtie2 (5/6) [2018-07-15 01:07:32] Mapping right_kept_reads.m2g_um_seg6 to genome genome with Bowtie2 (6/6) [2018-07-15 01:14:11] Searching for junctions via segment mapping [2018-07-15 13:29:29] Retrieving sequences for splices [2018-07-15 13:31:23] Indexing splices [2018-07-15 13:32:03] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/6) [2018-07-15 13:44:38] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/6) [2018-07-15 13:57:39] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/6) [2018-07-15 14:09:58] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/6) [2018-07-15 14:20:29] Mapping left_kept_reads.m2g_um_seg5 to genome segment_juncs with Bowtie2 (5/6) [2018-07-15 14:27:06] Mapping left_kept_reads.m2g_um_seg6 to genome segment_juncs with Bowtie2 (6/6) [2018-07-15 14:28:28] Joining segment hits [2018-07-15 14:37:05] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/6) [2018-07-15 14:50:37] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/6) [2018-07-15 15:04:35] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/6) [2018-07-15 15:17:26] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/6) [2018-07-15 15:28:25] Mapping right_kept_reads.m2g_um_seg5 to genome segment_juncs with Bowtie2 (5/6) [2018-07-15 15:35:28] Mapping right_kept_reads.m2g_um_seg6 to genome segment_juncs with Bowtie2 (6/6) [2018-07-15 15:37:10] Joining segment hits [2018-07-15 15:46:38] Reporting output tracks ----------------------------------------------- [2018-07-15 17:13:22] A summary of the alignment counts can be found in /scratch/6936766.1.linga/tophat2/align_summary.txt [2018-07-15 17:13:22] Run complete: 20:52:07 elapsed