@include "_basic_sc_rna_counter_stages.mro"

call FILTER_BARCODES(
    sample_id = "Nash_2_run",
    matrices_h5 = "/net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/NASH/data/NASH2/Nash_2_run/SC_RNA_COUNTER_CS/SC_RNA_COUNTER/_BASIC_SC_RNA_COUNTER/COUNT_GENES/fork0/join-u0fe2a74a09/files/matrices_h5.h5",
    barcode_correction_csv = "/net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/NASH/data/NASH2/Nash_2_run/SC_RNA_COUNTER_CS/SC_RNA_COUNTER/_BASIC_SC_RNA_COUNTER/MARK_DUPLICATES/fork0/join-u0fe2a74878/files/barcode_summary.csv",
    raw_fastq_summary = "/net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/NASH/data/NASH2/Nash_2_run/SC_RNA_COUNTER_CS/SC_RNA_COUNTER/_BASIC_SC_RNA_COUNTER/SUMMARIZE_READ_REPORTS/fork0/join-u0fe2a73613/files/summary.json",
    attach_bcs_summary = "/net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/NASH/data/NASH2/Nash_2_run/SC_RNA_COUNTER_CS/SC_RNA_COUNTER/_BASIC_SC_RNA_COUNTER/ATTACH_BCS_AND_UMIS/fork0/join-u0fe2a73f9e/files/summary.json",
    recovered_cells = null,
    force_cells = null,
    barcode_summary = "/net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/NASH/data/NASH2/Nash_2_run/SC_RNA_COUNTER_CS/SC_RNA_COUNTER/_BASIC_SC_RNA_COUNTER/COUNT_GENES/fork0/join-u0fe2a74a09/files/barcode_summary.h5",
    barcode_whitelist = "737K-august-2016",
    is_antibody_only = false,
    reference_path = "/restricted/projectnb/waxmanlab/kkarri/tabula_muris/drop-seq/mm10_ref/updated-usethis-mm10plus-pcg-ercc-lnc-nodups",
    gem_groups = [
        1,
        1,
        1,
        1,
        1,
        1,
        1,
        1,
        1,
        1,
        1,
        1,
        1,
        1,
        1,
        1,
        1,
        1,
        1,
        1,
        1,
        1,
        1,
        1,
        1,
        1,
        1,
        1,
        1,
        1,
        1,
        1,
        1,
        1,
        1,
        1,
        1
    ],
    chemistry_def = {
        "si_read_offset": 0,
        "strandedness": "+",
        "umi_read_offset": 16,
        "barcode_read_type": "R1",
        "umi_read_type": "R1",
        "barcode_read_length": 16,
        "rna_read_type": "R2",
        "barcode_whitelist": "737K-august-2016",
        "si_read_type": "I1",
        "rna_read_length": null,
        "description": "Single Cell 3' v2",
        "rna_read_offset": 0,
        "barcode_read_offset": 0,
        "rna_read2_type": null,
        "endedness": "three_prime",
        "name": "SC3Pv2",
        "umi_read_length": 10,
        "si_read_length": null,
        "read_type_to_bcl2fastq_filename": {
            "I1": "I1",
            "I2": null,
            "R1": "R1",
            "R2": "R2"
        },
        "rna_read2_length": null,
        "rna_read2_offset": 0,
        "read_type_to_bcl_processor_filename": {
            "I1": "I1",
            "I2": null,
            "R1": "RA",
            "R2": null
        }
    },
    cell_barcodes = null,
)